Figure 8

Mass balance analysis for separate hydrolysis and fermentation (SHF) (A), simultaneous saccharification and fermentation (SSF) (B), and SSF with 2% Tween 20 (C). For SHF, enzymatic hydrolysis was carried out at pH 4.8, 50°C, 200 rpm for 96 hours and fermentation of hydrolyzate was conducted at inoculation OD 4.0, 30°C, 50% nutrients, 200 rpm for 96 hours. For SSF, experiments were performed at 39°C, inoculation OD 4.0, pH 4.8, 50% nutrients, and 200 rpm for 192 hours. Data were collected from 12% (w/w) glucan loading experiments of SECS conversion. AIL, acid insoluble lignin; DW, dry weight; FPU, filter paper unit; OD, optical density; pNPGU, p-nitrophenol-β-D- glucopyranoside units; SECS, steam-exploded corn stover; SHF, separate hydrolysis and fermentation; SSF, simultaneous saccharification and fermentation.