Figure 2

Opimization of the high temp hold step. a: High temperature hold optimization. The digestion conditions are 50 mM acetate buffer, pH 5.5 with 100 mM NaCl, 15 mM CaCl₂ with enzyme loadings listed below. * Denotes a statistically (P < .05) relevant improvement in conversion compared to CTec2 at 120 h. High CelA mix: 5 mg C. bescii broth/g glucan, 1 mg E1/g glucan, 0.5 mg β-glucosidase/g glucan, and 13.5 mg CTec2/g glucan. Mid CelA mix: 3 mg C. bescii broth/g glucan, 1 mg E1/g glucan, 0.5 mg β-glucosidase/g glucan, and 15.5 mg CTec2/g glucan. Low CelA mix:* 2 mg C. bescii broth/g glucan, 1 mg E1/g glucan, 0.5 mg β-glucosidase/g glucan, and 16.5 mg CTec2/g glucan. Low E1 mix: 3 mg E1/g glucan, 0.5 mg β-glucosidase/g glucan, and 16.5 mg CTec2/g glucan. Mid E1 mix:* 4 mg E1/g glucan, 0.5 mg β-glucosidase/g glucan, and 15.5 mg CTec2/g glucan. High E1 mix: 6 mg E1/g glucan, 0.5 mg β-glucosidase/g glucan, and 13.5 mg CTec2/g glucan. CTec2: 20 mg CTec2/g glucan. b: High temperature hold optimization close-up of 48 h + digestion points to better illustrate differences.