Figure 3

Optimization of TAG biosynthesis in the MPS11 strain. A) Schematic representation of the plasmid systems used for modulating SCO0958 and lpp β genes expression. The gray dot upstream of the target genes represents Shine-Dalgarno sequences and the size of the bended arrows, the relative strength of the inducible P_T7 or P_BAD promoters. B) Cultures of E. coli MPS11 containing the different expression systems (1 to 4) were grown as described in Methods section, and expression of the respective genes was induced with L-arabinose and/or isopropyl-β-D-thiogalactopyranoside (IPTG). TAG content recovered from each of the cultures is expressed as percentage of cell dry weight mass (% CDW).