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Table 1 Strains and plasmids

From: Engineering a Streptomyces coelicolor biosynthesis pathway into Escherichia coli for high yield triglyceride production

Strain or plasmid Description Reference
Strains   
DH5α E. coli K12 F-lacU169 ( Φ80lacZM15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA1 [40]
BL21 (DE3) E. coli F-ompT gal dcm lon hsdS B (r B -m B -) λ(DE3) Novagen
MPS10 BL21 (DE3) ∆dgkA::Km; KmR This study
MPS11 BL21 (DE3) ∆dgkA This study
MPS12 BL21 (DE3) ∆dgkAfadE::Km; KmR This study
MPS13 BL21 (DE3) ∆dgkAfadE This study
MPS14 BL21 (DE3) ∆dgkAfadEaraBAD::Km; KmR This study
MPS15 BL21 (DE3) ∆dgkAfadEaraBAD This study
Plasmids   
pET28a Vector for expression of N-terminal His-tagged proteins under the strong T7 promoter; KmR Novagen
pBAD33 Vector for recombinant protein expression under the control of the P BAD promoter; CmR [41]
pKD13 Template plasmid for amplification of the FRT-flanked kanamycin cassette; ApR KmR [39]
pKD46 Temperature-sensitive replication plasmid for Red recombinase expression; ApR [39]
pCP20 Temperature-sensitive replication plasmid for thermal induction of FLP synthesis; CmR ApR [42]
pCR®-BluntII-TOPO Vector used for cloning of blunt PCR products; KmR Invitrogen
pBAD0958 pBAD33 carrying the SCO0958His gene under the control of P BAD promoter; CmR [15]
pET28-LPPβ pET28 carrying the SCO1753His gene under the control of T7 promoter; KmR [12]
pBAD-LPPβ pBAD33 carrying the SCO1753His gene under the control of P BAD promoter; CmR [12]
pTR257 pET28 carrying the SCO0958His gene under the control of T7 promoter; KmR [15]
pET28-0958- LPPβ pET28 carrying the SCO0958His-SCO1753His operon under the control of T7 promoter; KmR This study
pBAD-0958- LPPβ pBAD33 carrying the SCO0958His-SCO1753His operon under the control of P BAD promoter; CmR This study
pET28-AtfA pET28 carrying the atfAHis gene under the control of T7 promoter; KmR This study
pET28-FadD pET28 carrying the fadDHis gene under the control of T7 promoter; KmR This study
pET28-0958- FadD pET28 carrying the SCO0958His-fadDHis operon under the control of T7 promoter; KmR This study
pCC01 pET28 carrying the accA2His gene under the control of T7 promoter; KmR [43]
pET28-AccA2-AccE pET28 carrying the accA2His-accEHis operon under the control of T7 promoter; KmR This study
pET28-AccA2-AccE-AccB pET28 carrying the accA2His-accEHis-accBHis operon under the control of T7 promoter; KmR This study
pET28-0958-ACC pET28 carrying the SCO0958His-accA2His-accEHis-accBHis operon under the control of T7 promoter; KmR This study
pET28-GpsA pET28 carrying the gpsAHis gene under the control of T7 promoter; KmR This study
pET28-0958-GpsA pET28 carrying the SCO0958His-gpsAHis operon under the control of T7 promoter; KmR This study
pET28-FadR pET28 carrying the fadRHis gene under the control of T7 promoter; KmR This study
pET28-0958-FadR pET28 carrying the SCO0958His-fadRHis operon under the control of T7 promoter; KmR This study
pET28-0958-0958 pET28 carrying the SCO0958His-SCO0958His operon under the control of T7 promoter; KmR This study