Strains
| | |
DH5α
|
E. coli K12 F-lacU169 ( Φ80lacZ∆M15) endA1 recA1 hsdR17 deoR supE44 thi-1 l2 gyrA96 relA1
|
[40]
|
BL21 (DE3)
|
E. coli F-ompT gal dcm lon hsdS
B
(r
B
-m
B
-) λ(DE3)
|
Novagen
|
MPS10
|
BL21 (DE3) ∆dgkA::Km; KmR
|
This study
|
MPS11
|
BL21 (DE3) ∆dgkA
|
This study
|
MPS12
|
BL21 (DE3) ∆dgkA ∆fadE::Km; KmR
|
This study
|
MPS13
|
BL21 (DE3) ∆dgkA ∆fadE
|
This study
|
MPS14
|
BL21 (DE3) ∆dgkA ∆fadE ∆araBAD::Km; KmR
|
This study
|
MPS15
|
BL21 (DE3) ∆dgkA ∆fadE ∆araBAD
|
This study
|
Plasmids
| | |
pET28a
|
Vector for expression of N-terminal His-tagged proteins under the strong T7 promoter; KmR
|
Novagen
|
pBAD33
|
Vector for recombinant protein expression under the control of the P
BAD
promoter; CmR
|
[41]
|
pKD13
|
Template plasmid for amplification of the FRT-flanked kanamycin cassette; ApR KmR
|
[39]
|
pKD46
|
Temperature-sensitive replication plasmid for Red recombinase expression; ApR
|
[39]
|
pCP20
|
Temperature-sensitive replication plasmid for thermal induction of FLP synthesis; CmR ApR
|
[42]
|
pCR®-BluntII-TOPO
|
Vector used for cloning of blunt PCR products; KmR
|
Invitrogen
|
pBAD0958
|
pBAD33 carrying the SCO0958His gene under the control of P
BAD
promoter; CmR
|
[15]
|
pET28-LPPβ
|
pET28 carrying the SCO1753His gene under the control of T7 promoter; KmR
|
[12]
|
pBAD-LPPβ
|
pBAD33 carrying the SCO1753His gene under the control of P
BAD
promoter; CmR
|
[12]
|
pTR257
|
pET28 carrying the SCO0958His gene under the control of T7 promoter; KmR
|
[15]
|
pET28-0958- LPPβ
|
pET28 carrying the SCO0958His-SCO1753His operon under the control of T7 promoter; KmR
|
This study
|
pBAD-0958- LPPβ
|
pBAD33 carrying the SCO0958His-SCO1753His operon under the control of P
BAD
promoter; CmR
|
This study
|
pET28-AtfA
|
pET28 carrying the atfAHis gene under the control of T7 promoter; KmR
|
This study
|
pET28-FadD
|
pET28 carrying the fadDHis gene under the control of T7 promoter; KmR
|
This study
|
pET28-0958- FadD
|
pET28 carrying the SCO0958His-fadDHis operon under the control of T7 promoter; KmR
|
This study
|
pCC01
|
pET28 carrying the accA2His gene under the control of T7 promoter; KmR
|
[43]
|
pET28-AccA2-AccE
|
pET28 carrying the accA2His-accEHis operon under the control of T7 promoter; KmR
|
This study
|
pET28-AccA2-AccE-AccB
|
pET28 carrying the accA2His-accEHis-accBHis operon under the control of T7 promoter; KmR
|
This study
|
pET28-0958-ACC
|
pET28 carrying the SCO0958His-accA2His-accEHis-accBHis operon under the control of T7 promoter; KmR
|
This study
|
pET28-GpsA
|
pET28 carrying the gpsAHis gene under the control of T7 promoter; KmR
|
This study
|
pET28-0958-GpsA
|
pET28 carrying the SCO0958His-gpsAHis operon under the control of T7 promoter; KmR
|
This study
|
pET28-FadR
|
pET28 carrying the fadRHis gene under the control of T7 promoter; KmR
|
This study
|
pET28-0958-FadR
|
pET28 carrying the SCO0958His-fadRHis operon under the control of T7 promoter; KmR
|
This study
|
pET28-0958-0958
|
pET28 carrying the SCO0958His-SCO0958His operon under the control of T7 promoter; KmR
|
This study
|