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Table 2 PCR oligonucleotides

From: Engineering a Streptomyces coelicolor biosynthesis pathway into Escherichia coli for high yield triglyceride production

Name

Sequence (5′-3′)

Reference

dgkA_F

AGGATCTGCCGGAATAGACTTGCTT

This study

dgkA_R

AGCGCCTTCAGATGTTCTTCAGCC

This study

fadE_F

CAAAAGCGAGAAGTACGGGCAGGTG

This study

fadE_R

GCTTTCGATTGATGGTAAAACGGTG

This study

araBAD_F

CTCGATTTTGGCAGTGATTCTGTGCGAGCTTTGGCGGTGGCTGTCAAACATGAGAATTAA

This study

araBAD _R

CCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTGTGTAGGCTGGAGCTGCTTC

This study

ara_check_F

TAAACGAGTATCCCGGCAGCAGGGG

This study

ara_check _R

CGGGAATAAACGCCACGGACTCTTG

This study

araA_wt_F

AAGTGTATTACGGGTTTCGTCGCTA

This study

atfA_F

CATATG CGCCCATTACATCCGATTG

This study

atfA_R

ACTAGT CCTTTAGTTTTATCTGATA

This study

fadD_F

GCTAGC AAGAAGGTTTGGCTTAACC

This study

fadD_R

ACTAGT CTCAGGCTTTATTGTCCAC

This study

gpsA_F

CATATG AGCAAGCCGGTCAAGGCGG

This study

gpsA_R

ACTAGT GCGGGTTGCGCGGGGGGTC

This study

fadR_F

CATATG GTCATTAAGGCGCAAAGCC

This study

fadR_R

ACTAGT ATGGGAAATCTGTAAAAAC

This study

accB_F

CATATG ACCGTTTTGGATGAGGCGC

This study

accB_R

ACTAGT TCACTGCGGCGGGTTGCCG

This study

accE_F

CATATG TCCCCTGCCGACATCCGCG

This study

accE_R

ACTAGT TCAGCGCCAGCTGTGCGGG

This study

  1. Restriction sites are shown in italics. Homologous sequences to pKD13 plasmid are shown in bold.