Figure 6

TLC (a) and HPLC analysis (b) of hydrolysis products from colloidal chitin by the purified PbChi74 coupled with RmNAG. 5 U mL^-1 of PbChi74 (solid diamonds) or 1 U mL^-1 of RmNAG (open triangles) or 5 U mL^-1 of PbChi74 together with 1 U mL^-1 of RmNAG (solid circles) were added to 30 mg mL^-1 of colloidal chitin solution in 50 mM citrate buffer (pH 4.5), and incubated at 45°C for 24 h, separately. Aliquots taken at different times were analyzed by TLC and HPLC, respectively.