An acidic, thermostable exochitinase with β-N-acetylglucosaminidase activity from Paenibacillus barengoltzii converting chitin to N-acetyl glucosamine

Table 2 Purification summary of PbChi74 from P. barengoltzii

Purification step	Total activity (U)^a	Total protein (mg)^b	Specific activity (U mg^-1)	Purification factor (fold)	Recovery yield (%)
Crude enzyme	563.7	63.4	8.8	1	100
Ni-NTA agarose	326.6	16.4	19.9	2.2	57.9

^aEnzyme activity was measured at 65°C in 50 mM citrate buffer (pH 4.5) using 1% (w/v) of colloidal chitin as the substrate.
^bProtein concentration was measured by the Lowry method [41] using BSA as the standard.

ISSN: 2731-3654