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Table 3 Substrate specificity of the purified PbChi74 from P. barengoltzii

From: An acidic, thermostable exochitinase with β-N-acetylglucosaminidase activity from Paenibacillus barengoltzii converting chitin to N-acetyl glucosamine

Substrate Specific activity (U mg-1)a
Colloidal chitin 19.9 ± 0.2
Glycol chitin 22.2 ± 0.2
Powdery chitin 0.6 ± 0.1
CMC 0.5 ± 0.1
Chitosan b 0.3 ± 0.05
p NP-NAG 6.4 ± 0.1
(GlcNAc) 2 2.6 ± 0.3
(GlcNAc) 3 23.5 ± 0.8
(GlcNAc) 4 68.0 ± 2.1
(GlcNAc) 5 198.4 ± 7.9
  1. aThe enzyme activity was determined at 65°C in 50 mM sodium citrate buffer (pH 4.5).
  2. bThe degree of deacetylation of chitosan used is 85%.