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Table 4 Kinetic parameters of PbChi74 from P. barengoltzii a

From: An acidic, thermostable exochitinase with β-N-acetylglucosaminidase activity from Paenibacillus barengoltzii converting chitin to N-acetyl glucosamine

Substrate

Vmax(μmol min-1mg-1)

Km(mg mL-1)

kcat(s-1)

kcat/Km(mg-1s-1)

Colloidal chitin

23.22

2.4

0.028

0.011

Glycol chitin

23.4

1.84

0.029

0.016

  1. aThe kinetic parameters were determined at 65°C in 50 mM sodium citrate buffer (pH 4.5) for 5 min.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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