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Table 4 Kinetic parameters of PbChi74 from P. barengoltzii a

From: An acidic, thermostable exochitinase with β-N-acetylglucosaminidase activity from Paenibacillus barengoltzii converting chitin to N-acetyl glucosamine

Substrate Vmax(μmol min-1mg-1) Km(mg mL-1) kcat(s-1) kcat/Km(mg-1s-1)
Colloidal chitin 23.22 2.4 0.028 0.011
Glycol chitin 23.4 1.84 0.029 0.016
  1. aThe kinetic parameters were determined at 65°C in 50 mM sodium citrate buffer (pH 4.5) for 5 min.