Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification

Table 2 Hydrophobic patch score for full-length cellulases, individual catalytic domains (CDs), and carbohydrate binding modules (CBMs) for EG I, CBH I, and CBH II from Trichoderma reesei

	Unweighted hydrophobic patch score
Full-length EG I	6.8
Full-length CBH I	13.3
Full-length CBH II	27.9
EG I CD	6.2
CBH I CD	6.7
CBH II CD	23.7
EG I CBM	0.6
CBH I CBM	6.6
CBH II CBM	4.2

Structures were downloaded from the Protein Data Bank (PDB), modeled if necessary, and scored in Rosetta as described in the Methods section. Previous literature demonstrates that significant protein-lignin binding correlates with unweighted hydrophobic patch scores over 5 [25]. All full-length cellulases tested exceed this threshold.

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