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Table 5 Fermentation parameters for synthetic media (SM) in presence of various groups of lignocellulose decomposition products (DP) e

From: Designer synthetic media for studying microbial-catalyzed biofuel production

 

Biomass yield b (g/g)

Xylose consumption (%)

Ethanol productivity (g/L/h)

Ethanol yield c (g/g)

Glycerol yield (g/g)

Xylitol yield (g/g)

Acetate yield (g/g)

Carbon balance closure

18 h

24 h

48 h

24 h

48 h

48 h

48 h

48 h

48 h

Blank SMa

0.078 ± 0.002

66.92 ± 0.18

97.08 ± 0.04

1.29 ± 0.01

0.71 ± 0.01

0.406 ± 0.001

0.058 ± 0.000

0.053 ± 0.002

0.007 ± 0.000

1.00

Blank + Nitrogenous compounds

0.067 ± 0.003

44.69 ± 0.68

86.92 ± 0.61

1.21 ± 0.00

0.69 ± 0.01

0.402 ± 0.007

0.060 ± 0.001

0.055 ± 0.003

0.007 ± 0.000

0.98

Blank + Aliphatic acidsd

0.068 ± 0.002

58.95 ± 0.16

94.17 ± 0.04

1.23 ± 0.01

0.73 ± 0.01

0.427 ± 0.001

0.051 ± 0.000

0.042 ± 0.001

0.002 ± 0.000

1.01

Blank + Aromatic compounds

0.072 ± 0.002

68.70 ± 0.19

88.34 ± 0.04

1.29 ± 0.00

0.72 ± 0.01

0.421 ± 0.001

0.051 ± 0.000

0.032 ± 0.001

0.007 ± 0.000

1.00

Blank + Carbohydrates (oligos)

0.074 ± 0.002

54.68 ± 0.98

94.49 ± 0.50

1.28 ± 0.00

0.73 ± 0.00

0.416 ± 0.001

0.059 ± 0.000

0.043 ± 0.000

0.007 ± 0.000

1.01

Blank + Furans

0.078 ± 0.002

69.25 ± 0.19

96.90 ± 0.04

1.29 ± 0.00

0.72 ± 0.01

0.410 ± 0.001

0.059 ± 0.000

0.052 ± 0.002

0.007 ± 0.000

1.01

Blank + DP in combinationd

0.059 ± 0.002

21.17 ± 0.09

40.05 ± 1.28

1.20 ± 0.01

0.64 ± 0.00

0.440 ± 0.000

0.045 ± 0.000

0.024 ± 0.000

−0.002 ± 0.000

0.99

Actual Hydrolysated

0.065 ± 0.001

14.91 ± 0.53

43.31 ± 0.47

1.26 ± 0.01

0.73 ± 0.00

0.474 ± 0.002

0.048 ± 0.001

0.018 ± 0.000

−0.003 ± 0.001

1.06

  1. aThe blank was the synthetic medium without the addition of decomposition products (DP).
  2. bBiomass yield was based on both glucose and xylose consumed at 18 h fermentation, when the cell density reached the maximum. One unit of absorbance at 600 nm is approximately equal to 0.48 g dry cell wt/L.
  3. cTheoretical metabolic yield of ethanol for both sugars was 0.51 g EtOH/g consumed sugar.
  4. dThe initial concentration of acetate in the hydrolysate and synthetic medium with the addition of aliphatic acids and DP in combination was 1.9 g/L.
  5. eThe t-test results for determining statistically significant differences between the different results are presented in Additional file 1, S2, Tables S2-1 - S2-9.