Figure 5

Deacetylation by acetyl esterases from different carbohydrate esterase families. Distribution of acetyl moieties over 2-O, 3-O, and 2,3-di-O acetylated Xylp, and 3-O acetylated Xylp α-1,2 substituted with MeGlcA present in acetylated 4-O-methyl glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides (AcUXOS) from E. globulus and free acetic acid as proportion (%) of the sum of the total acetyl groups present after incubation of AcUXOS with acetyl esterases from different CE families (incubation time: 16 h). Structural units: (blue) -Xyl 3-O Ac 2-MeGlcA-, (red) -Xyl 2-O Ac-, (green) -Xyl 3-O Ac-, (purple) -Xyl 2,3-O Ac-, (gray) acetic acid; the control represents AcUXOS without enzyme treatment.