Positional preferences of acetyl esterases from different CE families towards acetylated 4-O-methyl glucuronic acid-substituted xylo-oligosaccharides

Table 2 Deacetylation rates and rates for acetic acid released

enzyme	-Xyl3- O Ac2-MeGlcA-	-Xyl2- O Ac-	-Xyl3- O Ac-	-Xyl2,3- O Ac-	Acetic acid
TeCE1^a	0.0	12.7	25.5	44.0	74.6
CtCE2^b	0.0	<0.01	6.5	0.0	5.7
CtCE3^b	0.0	<0.01	<0.01	0.0	<0.01
CtCE4^b	0.0	88.1	356.6	0.0	365.7
AnCE5^a	0.0	202.7	276.2	274.4	754.7
TrCE5^a	0.0	171.8	289.2	92.5	540.9
OsCE6^a	0.0	48.64	92.2	50.3	117.7
AnCE16^b	0.03	1.80	1.92	1.50	5.25

Deacetylation rates (μmol acetyl groups removed/min/mg enzyme) by acetyl esterases for 2-O, 3-O, and 2,3-di-O acetylated Xylp and 3-O acetylated Xylp 2-O substituted with MeGlcA from eucalyptus and rates for the total acetic acid released (μmol acetic acid released/min/mg enzyme).
^aμmol acetyl groups removed/min/mg enzyme and acetic acid released/min/mg enzyme after 5 min.
^bμmol acetyl groups removed/min/mg enzyme and acetic acid released/min/mg enzyme after 30 min.

ISSN: 2731-3654