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Table 2 Deacetylation rates and rates for acetic acid released

From: Positional preferences of acetyl esterases from different CE families towards acetylated 4-O-methyl glucuronic acid-substituted xylo-oligosaccharides

enzyme -Xyl3- O Ac2-MeGlcA- -Xyl2- O Ac- -Xyl3- O Ac- -Xyl2,3- O Ac- Acetic acid
TeCE1a 0.0 12.7 25.5 44.0 74.6
CtCE2b 0.0 <0.01 6.5 0.0 5.7
CtCE3b 0.0 <0.01 <0.01 0.0 <0.01
CtCE4b 0.0 88.1 356.6 0.0 365.7
AnCE5a 0.0 202.7 276.2 274.4 754.7
TrCE5a 0.0 171.8 289.2 92.5 540.9
OsCE6a 0.0 48.64 92.2 50.3 117.7
AnCE16b 0.03 1.80 1.92 1.50 5.25
  1. Deacetylation rates (μmol acetyl groups removed/min/mg enzyme) by acetyl esterases for 2-O, 3-O, and 2,3-di-O acetylated Xylp and 3-O acetylated Xylp 2-O substituted with MeGlcA from eucalyptus and rates for the total acetic acid released (μmol acetic acid released/min/mg enzyme).
  2. aμmol acetyl groups removed/min/mg enzyme and acetic acid released/min/mg enzyme after 5 min.
  3. bμmol acetyl groups removed/min/mg enzyme and acetic acid released/min/mg enzyme after 30 min.