The influences of ACh precursor and analogs on
U2-9. (a-c) Choline was more efficient for the dry weight, lipid content, and lipid productivity in comparison with ACh analogs. (d) The production of ALA was improved up to 321 ± 7 and 315 ± 5 mg g-1 of dry weight by choline and phosphatidylcholine, respectively. (e) The biodiesel yield was improved up to 36.5 ± 4% by 200 μg L-1 choline. Note: CHOL: choline, CHCL: choline chloride, CH: choline hydroxide, INN: citicoline, and PC: phosphatidylcholine. Samples with no drug treatments were used as control (Cont). All data were expressed as mean ± standard error (n = 3) of three separate tests.