Figure 3

Efficiency of ACh at dissimilar concentrations of Tris base and acetic acid. (a-c) Acetylcholine (ACh) enhanced dry weight, lipid content, and lipid productivity of C. sorokiniana U2-9 at dissimilar concentrations of Tris acetate. (d-e) The highest ALA (228.74 mg g^-1 of dry weight) and biodiesel yields (40.8%) were obtained at the level C of Tris acetate. Note: A-D describe the applied levels of Tris acetate. A: 1.2 g L^-1 Tris base and 5 mL L^-1glacial acetic acid, B: 2.4 g L^-1 Tris base and 10 mL L^-1 glacial acetic acid, C: 3.6 g L^-1 Tris base and 12.5 mL L^-1 glacial acetic acid, D: 4.8 g L^-1 Tris base and 15 mL L^-1 glacial acetic acid. All data were given as mean ± standard error (n = 3) of three separate tests.