Table 1 List of C. butyricum CWBI 1009-specific primers and probes used in this study
Target gene | Primer/probe | Sequence 5’ → 3’ | Length (bp) | Ref. |
|---|---|---|---|---|
recA | RecA-ButF | AAGCATTAGTGCGTTCTGGAG | 97 | [41] |
RecA-ButR | GAATCTCCCATTTCCCCTTC | |||
hydA2 | ButA2F | ATAGTTGCAATGGCTCCTGC | 250 | This study |
ButA2R | TTTCTGCTTGCCTAACCCAT | |||
hydA8 | ButA8F | TCTTTGGAGTTACAGGGGGA | 188 | This study |
ButA8R | TTCAGCATTTGCAAGACCAC | |||
hydB2 | ButB2F | TGGTGGTGTATCAACTGCTG | 168 | This study |
ButB2R | TTGCATCCCATTCCTTCAAT | |||
hydB3 | ButB3F | CAATGGTTGCTACAGGCAGA | 168 | This study |
ButB3R | CAAAAGCATCGAATAACGCA | |||
16S rRNA | 16SButF | CCTGCCTCATAGAGGGGAAT | 143 | This study |
16SButR | GAGCCGTTACCTCACCAACT | |||
HP16SButa | CCGCATAAGATTGTAGTACCGCATGGTACA | |||
nifH | NifHButF | CATCAGCATTGGCTGAGATG | 206 | This study |
NifHButR | TGGTTCTGGTCCTCCTGATT |