Figure 8

Phenotype of mutant Δ rca-1 versus wild type (WT) grown on corn straw. CS, corn straw; M, protein marker; YE, yeast extract. (A–D) Secreted proteins, mycelial dry weight (mycelia grown on 2% glucose, or sucrose for 16 h, and 2% corn straw for 7 days), SDS-PAGE, and enzymatic activities, including cellulose hydrolysis activity (CMCase), exo-glucanase activity (pNPCase), and xylanase activity, of Δrca-1 versus WT when grown for the indicated times. (E) Induction of selected CAZy genes in WT and Δrca-1 mutant after transfer 16-h sucrose grown mycelia to corn straw for 4 h. (F) Time course of relative expression levels of plant cell wall-degrading associated regulators in Δrca-1 mutant versus WT after transfer from 16-h sucrose culture to corn straw for the indicated times. Values represent the means of at least three biological replicates; error bars show standard deviation. Statistical significance was performed using a two-tailed Student’s t-test. *P < 0.05; **P < 0.01.