Table 3 Genes and primer sequences used for real-time PCR
Abbreviation | Gene name | Accession number | Primer forward | Primer reverse | Amplicon size (bp) |
|---|---|---|---|---|---|
accC | Acetyl-CoA carboxylase. biotin carboxylase subunit | KM364594 | CCAGGAAGAATAACCGCCTAC | GGGAGTCATAGTTTGGAGGAAC | 100 |
fatB | Fatty acyl-ACP thioesterase B | KM364595 | GGCCCAAATATGCCAACAATC | GACGATCCATCCAGCCTTAAA | 97 |
fatA | Fatty acyl-ACP thioesterase A | KM364596 | TGATGTTCGGGAAGAGCATTTA | GGAGCAGGATCTTCCAGTTTAG | 111 |
dgat1 | Diacylglycerol acyltransferase 1 | KM364597 | CAGCCTATGTGTCTGCTTCTAT | CTCGATCCACACCTTGAGATT | 98 |
pdat2 | Phospholipid:diacylglycerol acyltransferase 2 | KM364598 | TGATGACAGTGTGCCTGTATTG | CTTGTGCTGGTACTCCCTTATG | 115 |