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Table 2 The sugar composition of hydrolyzed C. homosphaera biomass after 48 h of enzymatic treatment

From: Untreated Chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases

Enzyme preparation Glucose Galactose Mannose Total yield (%)
  g/L Yield (%) g/L Yield (%) g/L Yield (%)
T. reesei 6.6 (0.0) 23.4 (0.1) 0.2 (0.0) 0.5 (0.0) 0.0 0.0 24.0 (0.1)
A. cellulolyticus 9.9 (0.4) 35.0 (1.3) 0.5 (0.0) 1.8 (0.0) 0.0 0.0 36.7 (1.3)
A. awamori 10.0 (0.2) 35.4 (0.6) 0.3 (0.0) 1.2 (0.0) 0.0 0.0 35.4 (0.6)
T. reesei-A. awamori 9.4 (0.2) 33.1 (0.7) 0.1 (0.0) 0.4 (0.0) 0.1 (0.0) 0.1 (0.0) 33.5 (0.7)
A. cellulolyticus-A. awamori a 11.4 (1.2) 40.2 (4.2) 0.6 (0.0) 2.0 (0.1) 0.0 (0.0) 0.1 (0.0) 42.4 (4.3)
β-Glucosidase 2.1 (0.1) 7.6 (0.3) 0.1 (0.0) 0.3 (0.0) 0.0 0.0 7.9 (0.3)
Control 1.2 (0.1) 4.2 (0.3) 0.1 (0.0) 0.4 (0.0) 0.0 0.0 4.6 (0.4)
  1. The results are shown as the sugar concentration (g/L) and hydrolysis yield in terms of the total sugar concentration (%). The hydrolysis and HPAEC-PAD analysis conditions are described in the ‘Materials and methods’ section. As a control, the sugar released from the biomass that was incubated without any enzyme preparation is also shown. Numbers shown in brackets are standard deviations of the means.
  2. aXylose and arabinose at a low concentration (0.03 g/L; 0.12% yield) were released solely for the hydrolysis performed by the A. cellulolyticus-A. awamori enzyme blend.