Skip to main content

Advertisement

Table 3 Predicted kinetic parameters for C. homosphaera enzymatic hydrolysis yields

From: Untreated Chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases

Enzyme preparation Predicted parameters
Hexose equivalents (SE) Glucose (SE)
V 0 (mg/g/h) P max (mg/g) t ½ (h) V 0 (mg/g/h) P max (mg/g) t ½ (h)
T. reesei a 27.5 (6.3) 256.8 (38.9) 12.9 33.7 (4.3) 255.56 (40.2) 5.9
A. cellulolyticus 58.8 (2.1) 336.2 (3.2) 4.0 61.82 (2.7) 311.1 (3.5) 3.5
A. awamori 114.3 (9.0) 311.7 (9.0) 1.9 140.3 (16.9) 318.2 (6.3) 1.6
T. reesei and A. awamori 115.8 (16.1) 335.4 (27.3) 2.2 149.8 (29.2) 348.0 (11.3) 1.6
A. cellulolyticus and A. awamori 185.6 (16.7) 494.6 (8.6) 1.8 103.5 (7.9) 470.8 (9.3) 3.2
β-Glucosidase 8.4 (0.4) 88.0 (1.9) 7.3 8.9 (0.5) 116.0 (2.6) 9.0
Control 3.1 (0.1) 33.6 (0.5) 7.4 2.5 (0.3) 37.3 (1.5) 10.4
  1. The data were fitted into an exponential model as described in the materials and methods, and the parameters were estimated as follows: the V 0 was estimated by the limit of the first derivative of the fitting function when time → 0, P max was estimated by the limit of the fitting function when time → ∞, t½ was estimated using the curve fitting model by calculating the predicted P max/2. The number in brackets accounts for the standard error of the estimation.
  2. aExperimental results were used for the calculations because the parameters predicted by the fitting function showed very large standard errors. The V 0 was calculated using the first experimental result, i.e., the hydrolysis yield after two hours of incubation. The P max was estimated using the 48 h hydrolysis yield. The t½ was estimated by calculating the P max/2 and estimating the corresponding time using the fitting function.