Enzyme preparation

Predicted parameters


Hexose equivalents (SE)

Glucose (SE)


V
_{0}
(mg/g/h)

P
_{max}
(mg/g)

t
½ (h)

V
_{0}
(mg/g/h)

P
_{max}
(mg/g)

t
½ (h)


T. reesei
^{a}

27.5 (6.3)

256.8 (38.9)

12.9

33.7 (4.3)

255.56 (40.2)

5.9

A. cellulolyticus

58.8 (2.1)

336.2 (3.2)

4.0

61.82 (2.7)

311.1 (3.5)

3.5

A. awamori

114.3 (9.0)

311.7 (9.0)

1.9

140.3 (16.9)

318.2 (6.3)

1.6

T. reesei and A. awamori

115.8 (16.1)

335.4 (27.3)

2.2

149.8 (29.2)

348.0 (11.3)

1.6

A. cellulolyticus and A. awamori

185.6 (16.7)

494.6 (8.6)

1.8

103.5 (7.9)

470.8 (9.3)

3.2

βGlucosidase

8.4 (0.4)

88.0 (1.9)

7.3

8.9 (0.5)

116.0 (2.6)

9.0

Control

3.1 (0.1)

33.6 (0.5)

7.4

2.5 (0.3)

37.3 (1.5)

10.4

 The data were fitted into an exponential model as described in the materials and methods, and the parameters were estimated as follows: the V
_{0} was estimated by the limit of the first derivative of the fitting function when time → 0, P
_{max} was estimated by the limit of the fitting function when time → ∞, t½ was estimated using the curve fitting model by calculating the predicted P
_{max}/2. The number in brackets accounts for the standard error of the estimation.

^{a}Experimental results were used for the calculations because the parameters predicted by the fitting function showed very large standard errors. The V
_{0} was calculated using the first experimental result, i.e., the hydrolysis yield after two hours of incubation. The P
_{max} was estimated using the 48 h hydrolysis yield. The t½ was estimated by calculating the P
_{max}/2 and estimating the corresponding time using the fitting function.