Table 4 The predicted kinetic parameters for C. homosphaera enzymatic hydrolysis yields at a 1.5 IU/g endoglucanase load and final β-glucosidase loads of 7.5, 15.0 and 22.5 IU/g
Enzyme preparation | β-Glucosidase load (IU/g dry biomass) | Predicted parameters | |||||
|---|---|---|---|---|---|---|---|
Hexose equivalents (SE) | Glucose (SE) | ||||||
V 0 (mg/g/h) | P max (mg/g) | t ½ (h) | V 0 (mg/g/h) | P max (mg/g) | t ½ (h) | ||
T. reesei | 7.5 | 48.2 (7.5) | 203.3 (10.1) | 9.5 | 59.1 (17.5) | 184.8 (9.2) | 9.3 |
15.0 | 61.0 (16.9) | 286.6 (19.1) | 9.6 | 78.0 (17.8) | 228.0 (8.9) | 9.0 | |
22.5 | 93.4 (13.8) | 275.1 (19.4) | 6.8 | 91.9 (8.5) | 253.5 (3.1) | 7.8 | |
A. cellulolyticus | 7.5 | 53.2 (5.9) | 176.0 (5.7) | 6.5 | 59.4 (7.9) | 190.7 (12.8) | 6.4 |
15.0 | 95.5 (13.1) | 254.8 (11.6) | 7.8 | 96.5 (10.6) | 241.8 (12.2) | 8.2 | |
22.5 | 98.3 (12.7) | 271.1 (14.4) | 5.6 | 129.9 (32.7) | 292.2 (4.6) | 8.2 | |
A. awamori | 7.5 | 165.3 (24.7) | 273.8 (20,7) | 1.5 | 239.4 (100.3) | 249.4 (12.8) | 1.0 |
15.0 | 176.8 (29.6) | 325.8 (14.0) | 1.9 | 254.6 (181.1) | 314.2 (15.1) | 2.2 | |
22.5 | 158.5 (6.8) | 452.5 (24.2) | 3.6 | 246.9 (477.9) | 329.2 (39.8) | 2.4 | |
T. reesei and A. awamori | 7.5 | 63.9 (9.2) | 226.0 (9.0) | 4.2 | 71.6 (20.8) | 226.3 (8.1) | 8.2 |
15.0 | 74.1 (15,2) | 250.5 (12.4) | 4.4 | 69.3 (17.3) | 251.14 (12.0) | 7.8 | |
22.5 | 95.6 (25.1) | 290.6 (13.7) | 5.3 | 88.2 (26.4) | 248.7 (9.2) | 12.4 | |