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Table 4 The predicted kinetic parameters for C. homosphaera enzymatic hydrolysis yields at a 1.5 IU/g endoglucanase load and final β-glucosidase loads of 7.5, 15.0 and 22.5 IU/g

From: Untreated Chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases

Enzyme preparation β-Glucosidase load (IU/g dry biomass) Predicted parameters
Hexose equivalents (SE) Glucose (SE)
V 0 (mg/g/h) P max (mg/g) t ½ (h) V 0 (mg/g/h) P max (mg/g) t ½ (h)
T. reesei 7.5 48.2 (7.5) 203.3 (10.1) 9.5 59.1 (17.5) 184.8 (9.2) 9.3
15.0 61.0 (16.9) 286.6 (19.1) 9.6 78.0 (17.8) 228.0 (8.9) 9.0
22.5 93.4 (13.8) 275.1 (19.4) 6.8 91.9 (8.5) 253.5 (3.1) 7.8
A. cellulolyticus 7.5 53.2 (5.9) 176.0 (5.7) 6.5 59.4 (7.9) 190.7 (12.8) 6.4
15.0 95.5 (13.1) 254.8 (11.6) 7.8 96.5 (10.6) 241.8 (12.2) 8.2
22.5 98.3 (12.7) 271.1 (14.4) 5.6 129.9 (32.7) 292.2 (4.6) 8.2
A. awamori 7.5 165.3 (24.7) 273.8 (20,7) 1.5 239.4 (100.3) 249.4 (12.8) 1.0
15.0 176.8 (29.6) 325.8 (14.0) 1.9 254.6 (181.1) 314.2 (15.1) 2.2
22.5 158.5 (6.8) 452.5 (24.2) 3.6 246.9 (477.9) 329.2 (39.8) 2.4
T. reesei and A. awamori 7.5 63.9 (9.2) 226.0 (9.0) 4.2 71.6 (20.8) 226.3 (8.1) 8.2
15.0 74.1 (15,2) 250.5 (12.4) 4.4 69.3 (17.3) 251.14 (12.0) 7.8
22.5 95.6 (25.1) 290.6 (13.7) 5.3 88.2 (26.4) 248.7 (9.2) 12.4
  1. The data were fitted into an exponential model as described in the ‘Materials and methods’ section, and the parameters were estimated as described in Table 3. The numbers in brackets account for the standard error of the estimation.