Growth curves of
NCYC 2826 measured by optical density at 600 nm. Data shown are the average of three replicate experiments. (A,
C) Growth using 10% wheat straw hydrolysate only (squares), 10% wheat straw hydrolysate and YNB (circles), or 10% wheat straw hydrolysate and 2.3 mg ml−1 urea (triangles). (B,
D) Growth in media containing 2.3 mg ml−1 urea and initial wheat straw concentrations of 5% (squares), 10% (closed circles), 15% (triangle) or 20% (open circle). OD, optical density; hr, hour.