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Table 4 Glycosyl residue composition of cell wall (AIR) fractions from stem of controls and PdGAUT12.1 -KD plants

From: Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

   Sugar composition of cell wall fractions (mol %; ± SE standard error)
  Ara Rha Fuc Xyl GlcA GalA Man Gal Glc
Ammonium oxalate
WT 20.9 ± 1.0 4.1 ± 0.2 0.7 ± 0.02 8.9 ± 0.2 0.6 ± 0.01 23.1 ± 1.0 11.4 ± 0.9 3.7 ± 0.3 26.8 ± 1.2
V Control-1 21.0 ± 0.6 4.9 ± 0.3 0.8 ± 0.02 8.3 ± 0.4 0.5 ± 0.02 23.2 ± 1.1 11.6 ± 1.0 3.6 ± 0.2 26.9 ± 1.0
AB30.1 18.5 ± 0.4 1.0 ± 0.3** 0.2 ± 0.01 10.2 ± 0.5 0.7 ± 0.02 14.2 ± 1.3* 19.5 ± 1.1* 5.6 ± 0.3* 30.6 ± 0.9*
AB30.3 17.7 ± 0.6* 2.1 ± 0.2* 0.4 ± 0.01 10.5 ± 0.2* 0.7 ± 0.01 12.9 ± 0.9** 18.2 ± 1.3* 6.0 ± 0.1* 31.4 ± 1.0*
AB30.8 19.8 ± 0.8 2.1 ± 0.1* 0.4 ± 0.01 11.1 ± 0.3* 0.8 ± 0.01 5.0 ± 0.5** 21.9 ± 1.2* 6.2 ± 0.3* 32.8 ± 1.1*
AB30.11 19.3 ± 0.6 2.3 ± 0.2* 0.4 ± 0.02 11.4 ± 0.3* 0.8 ± 0.01 8.9 ± 0.8** 20.2 ± 1.3* 7.1 ± 0.2* 29.4 ± 1.2
Sodium carbonate
WT 10.7 ± 0.6 6.0 ± 0.2 0.3 ± 0.02 23.0 ± 0.2 1.6 ± 0.01 19.3 ± 0.5 14.0 ± 0.9 7.0 ± 0.3 18.0 ± 0.9
V Control-1 11.1 ± 0.4 5.9 ± 0.1 0.5 ± 0.02 22.5 ± 0.6 1.5 ± 0.02 19.5 ± 0.8 15.0 ± 0.8 6.7 ± 0.2 17.9 ± 0.7
AB30.1 4.8 ± 0.4* 2.7 ± 0.2* 0.4 ± 0.01 24.2 ± 0.5 1.7 ± 0.02 16.0 ± 0.3* 18.0 ± 1.0 5.8 ± 0.3 26.9 ± 0.9*
AB30.3 7.3 ± 0.3 2.7 ± 0.1* 0.3 ± 0.01 24.7 ± 0.2 1.7 ± 0.01 14.0 ± 0.4* 17.2 ± 0.6 6.8 ± 0.3 25.0 ± 1.0*
AB30.8 7.9 ± 0.3 3.7 ± 0.2* 0.8 ± 0.01 26.2 ± 0.3 2.6 ± 0.01 11.2 ± 0.5* 19.3 ± 0.9 6.7 ± 0.4 21.9 ± 1.1
AB30.11 7.3 ± 0.4 3.4 ± 0.3* 0.4 ± 0.02 25.7 ± 0.3 1.9 ± 0.01 13.5 ± 0.4* 16.3 ± 0.7 7.4 ± 0.2 24.2 ± 1.2*
1 M KOH
WT 0.5 ± 0.04 4.2 ± 0.2 0.2 ± 0.02 69.3 ± 1.9 8.5 ± 0.3 3.3 ± 0.1 6.4 ± 0.6 1.7 ± 0.2 7.4 ± 1.2
V Control-1 0.6 ± 0.03 4.1 ± 0.1 0.3 ± 0.01 70.1 ± 2.3 8.7 ± 0.2 3.4 ± 0.2 6.0 ± 0.5 1.6 ± 0.3 7.1 ± 1.4
AB30.1 1.1 ± 0.05 3.1 ± 0.2 0.4 ± 0.01 44.8 ± 2.3* 5.5 ± 0.2* 1.3 ± 0.09* 10.7 ± 0.5* 4.1 ± 0.3** 29.3 ± 2.1**
AB30.3 1.6 ± 0.06 3.1 ± 0.1 0.4 ± 0.01 42.4 ± 2.4* 5.3 ± 0.3* 1.2 ± 0.1* 11.1 ± 1.0* 3.7 ± 0.1** 31.9 ± 2.5**
AB30.8 1.8 ± 0.1 2.6 ± 0.1* 0.5 ± 0.01 37.8 ± 2.6** 4.7 ± 0.4* 0.8 ± 0.08** 14.6 ± 0.8* 4.8 ± 0.2** 33.2 ± 2.1**
AB30.11 1.6 ± 0.06 3.3 ± 0.2 0.4 ± 0.02 38.4 ± 3.2** 4.8 ± 0.3* 1.0 ± 0.1** 11.2 ± 0.4* 3.8 ± 0.2** 35.9 ± 2.4**
4 M KOH
WT 2.3 ± 0.08 3.0 ± 0.1 0.5 ± 0.02 37.8 ± 1.3 4.7 ± 0.2 0.8 ± 0.04 11.9 ± 1.2 7.8 ± 0.3 31.2 ± 1.2
V Control-1 2.4 ± 0.06 3.2 ± 0.2 0.3 ± 0.01 38.1 ± 1.5 4.9 ± 0.2 0.9 ± 0.03 11.4 ± 0.5 7.9 ± 0.3 30.8 ± 1.5
AB30.1 1.6 ± 0.09 2.4 ± 0.1 0.4 ± 0.01 26.2 ± 1.7* 3.4 ± 0.2* 0.5 ± 0.03* 18.2 ± 0.5** 8.4 ± 0.3 38.9 ± 1.8*
AB30.3 2.1 ± 0.07 2.2 ± 0.2 0.3 ± 0.01 25.2 ± 1.1* 3.3 ± 0.3* 0.4 ± 0.02* 15.6 ± 1.0* 10.9 ± 0.5** 39.9 ± 1.5*
AB30.8 1.7 ± 0.05 1.9 ± 0.2* 0.3 ± 0.01 22.3 ± 1.3** 2.7 ± 0.4* 0.3 ± 0.03** 18.9 ± 0.8** 8.6 ± 0.6* 43.2 ± 1.3**
AB30.11 1.9 ± 0.09 2.3 ± 0.1 0.4 ± 0.02 23.2 ± 1.4** 2.9 ± 0.3* 0.3 ± 0.05** 15.9 ± 0.4* 8.1 ± 0.2 44.9 ± 1.9**
Chlorite
WT 7.2 ± 0.5 4.3 ± 0.1 - 10.9 ± 0.5 0 6.4 ± 0.2 2.1 ± 0.1 11.2 ± 0.4 57.9 ± 1.1
V Control-1 7.0 ± 0.3 4.4 ± 0.1 0.2 ± 0.02 11.1 ± 0.7 0 6.5 ± 0.3 2.2 ± 0.09 10.9 ± 0.5 57.6 ± 1.0
AB30.1 5.5 ± 0.3* 2.7 ± 0.2* - 7.2 ± 0.4* 0 4.9 ± 0.1* 3.0 ± 0.1** 13.6 ± 0.6* 63.1 ± 0.9*
AB30.3 5.1 ± 0.3* 2.7 ± 0.3* - 7.0 ± 0.5* 0 4.6 ± 0.2* 3.2 ± 0.08** 14.5 ± 0.3* 63.6 ± 1.2*
AB30.8 4.5 ± 0.4* 3.7 ± 0.2* - 5.8 ± 0.6** 0 3.4 ± 0.3* 3.1 ± 0.09** 14.2 ± 0.4* 64.9 ± 1.4*
AB30.11 4.4 ± 0.3* 3.4 ± 0.1* 0.4 ± 0.01 6.2 ± 0.7* 0 4.3 ± 0.2* 3.6 ± 0.1** 14.1 ± 0.5* 63.7 ± 0.9*
4 M KOH PC
WT 1.1 ± 0.05 2.5 ± 0.10 - 65.1 ± 2.1 7.9 ± 0.2 2.4 ± 0.06 6.2 ± 1.2 5.0 ± 0.8 10.1 ± 0.9
V Control-1 1.3 ± 0.06 2.4 ± 0.09 - 64.6 ± 2.3 7.8 ± 0.2 2.5 ± 0.07 6.3 ± 1.0 5.2 ± 0.6 10.3 ± 1.0
AB30.1 1.1 ± 0.04 2.4 ± 0.08 - 47.5 ± 1.9* 5.9 ± 0.2* 2.2 ± 0.06 13.2 ± 1.3* 9.1 ± 0.7* 18.8 ± 1.1**
AB30.3 1.0 ± 0.03 2.2 ± 0.06 - 42.3 ± 1.5* 5.2 ± 0.3* 2.1 ± 0.06 14.6 ± 1.4** 10.7 ± 0.9** 21.8 ± 1.2**
AB30.8 0.9 ± 0.05 2.1 ± 0.07 - 35.7 ± 1.8** 4.3 ± 0.2** 2.0 ± 0.08 17.9 ± 1.7** 12.4 ± 1.0** 24.6 ± 1.3**
AB30.11 1.0 ± 0.05 2.2 ± 0.09 - 37.8 ± 1.6** 4.7 ± 0.3** 2.0 ± 0.09 18.5 ± 0.8** 11.8 ± 0.5** 22.2 ± 0.9**
  1. The amount of sugar is represented as average mol% in each wall extract. AIR (alcohol insoluble residue) was sequentially extracted using 50 mM ammonium oxalate, 50 mM Na2CO3, 1 M KOH, 4 M KOH, 100 mM sodium chlorite (chlorite) and 4 M KOH PC (post chlorite), hydrolyzed with methanolic HCl, and analyzed by GC. Data are mean ± SE of three biological and two technical replicates. Ital values represent significant difference between WT and PdGAUT12.1-KD plants at *P ≤ 0.05, **P ≤ 0.001 significance level (one-way ANOVA followed by Tukey’s multiple comparison test). A dash designates that the amount of sugar was below detection limits.