Skip to main content

Table 4 Glycosyl residue composition of cell wall (AIR) fractions from stem of controls and PdGAUT12.1 -KD plants

From: Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

  

Sugar composition of cell wall fractions (mol %; ± SE standard error)

 

Ara

Rha

Fuc

Xyl

GlcA

GalA

Man

Gal

Glc

Ammonium oxalate

WT

20.9 ± 1.0

4.1 ± 0.2

0.7 ± 0.02

8.9 ± 0.2

0.6 ± 0.01

23.1 ± 1.0

11.4 ± 0.9

3.7 ± 0.3

26.8 ± 1.2

V Control-1

21.0 ± 0.6

4.9 ± 0.3

0.8 ± 0.02

8.3 ± 0.4

0.5 ± 0.02

23.2 ± 1.1

11.6 ± 1.0

3.6 ± 0.2

26.9 ± 1.0

AB30.1

18.5 ± 0.4

1.0 ± 0.3**

0.2 ± 0.01

10.2 ± 0.5

0.7 ± 0.02

14.2 ± 1.3*

19.5 ± 1.1*

5.6 ± 0.3*

30.6 ± 0.9*

AB30.3

17.7 ± 0.6*

2.1 ± 0.2*

0.4 ± 0.01

10.5 ± 0.2*

0.7 ± 0.01

12.9 ± 0.9**

18.2 ± 1.3*

6.0 ± 0.1*

31.4 ± 1.0*

AB30.8

19.8 ± 0.8

2.1 ± 0.1*

0.4 ± 0.01

11.1 ± 0.3*

0.8 ± 0.01

5.0 ± 0.5**

21.9 ± 1.2*

6.2 ± 0.3*

32.8 ± 1.1*

AB30.11

19.3 ± 0.6

2.3 ± 0.2*

0.4 ± 0.02

11.4 ± 0.3*

0.8 ± 0.01

8.9 ± 0.8**

20.2 ± 1.3*

7.1 ± 0.2*

29.4 ± 1.2

Sodium carbonate

WT

10.7 ± 0.6

6.0 ± 0.2

0.3 ± 0.02

23.0 ± 0.2

1.6 ± 0.01

19.3 ± 0.5

14.0 ± 0.9

7.0 ± 0.3

18.0 ± 0.9

V Control-1

11.1 ± 0.4

5.9 ± 0.1

0.5 ± 0.02

22.5 ± 0.6

1.5 ± 0.02

19.5 ± 0.8

15.0 ± 0.8

6.7 ± 0.2

17.9 ± 0.7

AB30.1

4.8 ± 0.4*

2.7 ± 0.2*

0.4 ± 0.01

24.2 ± 0.5

1.7 ± 0.02

16.0 ± 0.3*

18.0 ± 1.0

5.8 ± 0.3

26.9 ± 0.9*

AB30.3

7.3 ± 0.3

2.7 ± 0.1*

0.3 ± 0.01

24.7 ± 0.2

1.7 ± 0.01

14.0 ± 0.4*

17.2 ± 0.6

6.8 ± 0.3

25.0 ± 1.0*

AB30.8

7.9 ± 0.3

3.7 ± 0.2*

0.8 ± 0.01

26.2 ± 0.3

2.6 ± 0.01

11.2 ± 0.5*

19.3 ± 0.9

6.7 ± 0.4

21.9 ± 1.1

AB30.11

7.3 ± 0.4

3.4 ± 0.3*

0.4 ± 0.02

25.7 ± 0.3

1.9 ± 0.01

13.5 ± 0.4*

16.3 ± 0.7

7.4 ± 0.2

24.2 ± 1.2*

1 M KOH

WT

0.5 ± 0.04

4.2 ± 0.2

0.2 ± 0.02

69.3 ± 1.9

8.5 ± 0.3

3.3 ± 0.1

6.4 ± 0.6

1.7 ± 0.2

7.4 ± 1.2

V Control-1

0.6 ± 0.03

4.1 ± 0.1

0.3 ± 0.01

70.1 ± 2.3

8.7 ± 0.2

3.4 ± 0.2

6.0 ± 0.5

1.6 ± 0.3

7.1 ± 1.4

AB30.1

1.1 ± 0.05

3.1 ± 0.2

0.4 ± 0.01

44.8 ± 2.3*

5.5 ± 0.2*

1.3 ± 0.09*

10.7 ± 0.5*

4.1 ± 0.3**

29.3 ± 2.1**

AB30.3

1.6 ± 0.06

3.1 ± 0.1

0.4 ± 0.01

42.4 ± 2.4*

5.3 ± 0.3*

1.2 ± 0.1*

11.1 ± 1.0*

3.7 ± 0.1**

31.9 ± 2.5**

AB30.8

1.8 ± 0.1

2.6 ± 0.1*

0.5 ± 0.01

37.8 ± 2.6**

4.7 ± 0.4*

0.8 ± 0.08**

14.6 ± 0.8*

4.8 ± 0.2**

33.2 ± 2.1**

AB30.11

1.6 ± 0.06

3.3 ± 0.2

0.4 ± 0.02

38.4 ± 3.2**

4.8 ± 0.3*

1.0 ± 0.1**

11.2 ± 0.4*

3.8 ± 0.2**

35.9 ± 2.4**

4 M KOH

WT

2.3 ± 0.08

3.0 ± 0.1

0.5 ± 0.02

37.8 ± 1.3

4.7 ± 0.2

0.8 ± 0.04

11.9 ± 1.2

7.8 ± 0.3

31.2 ± 1.2

V Control-1

2.4 ± 0.06

3.2 ± 0.2

0.3 ± 0.01

38.1 ± 1.5

4.9 ± 0.2

0.9 ± 0.03

11.4 ± 0.5

7.9 ± 0.3

30.8 ± 1.5

AB30.1

1.6 ± 0.09

2.4 ± 0.1

0.4 ± 0.01

26.2 ± 1.7*

3.4 ± 0.2*

0.5 ± 0.03*

18.2 ± 0.5**

8.4 ± 0.3

38.9 ± 1.8*

AB30.3

2.1 ± 0.07

2.2 ± 0.2

0.3 ± 0.01

25.2 ± 1.1*

3.3 ± 0.3*

0.4 ± 0.02*

15.6 ± 1.0*

10.9 ± 0.5**

39.9 ± 1.5*

AB30.8

1.7 ± 0.05

1.9 ± 0.2*

0.3 ± 0.01

22.3 ± 1.3**

2.7 ± 0.4*

0.3 ± 0.03**

18.9 ± 0.8**

8.6 ± 0.6*

43.2 ± 1.3**

AB30.11

1.9 ± 0.09

2.3 ± 0.1

0.4 ± 0.02

23.2 ± 1.4**

2.9 ± 0.3*

0.3 ± 0.05**

15.9 ± 0.4*

8.1 ± 0.2

44.9 ± 1.9**

Chlorite

WT

7.2 ± 0.5

4.3 ± 0.1

-

10.9 ± 0.5

0

6.4 ± 0.2

2.1 ± 0.1

11.2 ± 0.4

57.9 ± 1.1

V Control-1

7.0 ± 0.3

4.4 ± 0.1

0.2 ± 0.02

11.1 ± 0.7

0

6.5 ± 0.3

2.2 ± 0.09

10.9 ± 0.5

57.6 ± 1.0

AB30.1

5.5 ± 0.3*

2.7 ± 0.2*

-

7.2 ± 0.4*

0

4.9 ± 0.1*

3.0 ± 0.1**

13.6 ± 0.6*

63.1 ± 0.9*

AB30.3

5.1 ± 0.3*

2.7 ± 0.3*

-

7.0 ± 0.5*

0

4.6 ± 0.2*

3.2 ± 0.08**

14.5 ± 0.3*

63.6 ± 1.2*

AB30.8

4.5 ± 0.4*

3.7 ± 0.2*

-

5.8 ± 0.6**

0

3.4 ± 0.3*

3.1 ± 0.09**

14.2 ± 0.4*

64.9 ± 1.4*

AB30.11

4.4 ± 0.3*

3.4 ± 0.1*

0.4 ± 0.01

6.2 ± 0.7*

0

4.3 ± 0.2*

3.6 ± 0.1**

14.1 ± 0.5*

63.7 ± 0.9*

4 M KOH PC

WT

1.1 ± 0.05

2.5 ± 0.10

-

65.1 ± 2.1

7.9 ± 0.2

2.4 ± 0.06

6.2 ± 1.2

5.0 ± 0.8

10.1 ± 0.9

V Control-1

1.3 ± 0.06

2.4 ± 0.09

-

64.6 ± 2.3

7.8 ± 0.2

2.5 ± 0.07

6.3 ± 1.0

5.2 ± 0.6

10.3 ± 1.0

AB30.1

1.1 ± 0.04

2.4 ± 0.08

-

47.5 ± 1.9*

5.9 ± 0.2*

2.2 ± 0.06

13.2 ± 1.3*

9.1 ± 0.7*

18.8 ± 1.1**

AB30.3

1.0 ± 0.03

2.2 ± 0.06

-

42.3 ± 1.5*

5.2 ± 0.3*

2.1 ± 0.06

14.6 ± 1.4**

10.7 ± 0.9**

21.8 ± 1.2**

AB30.8

0.9 ± 0.05

2.1 ± 0.07

-

35.7 ± 1.8**

4.3 ± 0.2**

2.0 ± 0.08

17.9 ± 1.7**

12.4 ± 1.0**

24.6 ± 1.3**

AB30.11

1.0 ± 0.05

2.2 ± 0.09

-

37.8 ± 1.6**

4.7 ± 0.3**

2.0 ± 0.09

18.5 ± 0.8**

11.8 ± 0.5**

22.2 ± 0.9**

  1. The amount of sugar is represented as average mol% in each wall extract. AIR (alcohol insoluble residue) was sequentially extracted using 50 mM ammonium oxalate, 50 mM Na2CO3, 1 M KOH, 4 M KOH, 100 mM sodium chlorite (chlorite) and 4 M KOH PC (post chlorite), hydrolyzed with methanolic HCl, and analyzed by GC. Data are mean ± SE of three biological and two technical replicates. Ital values represent significant difference between WT and PdGAUT12.1-KD plants at *P ≤ 0.05, **P ≤ 0.001 significance level (one-way ANOVA followed by Tukey’s multiple comparison test). A dash designates that the amount of sugar was below detection limits.
Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us