Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

Table 5 Glycosyl linkage analysis of fractionated cell wall fractions from wild type P. deltoides and PdGAUT12.1 -KD lines

Fractions	Ammonium oxalate soluble			Na ₂ CO ₃ soluble			1 M KOH soluble
Fractions	WT	AB30.1	AB30.8	WT	AB30.1	AB30.8	WT	AB30.1	AB30.8
t-Araf	3.6	1.5	3.3	3.9	1.2	2.6	0.2	0.6	0.9
t-Arap	0.2	0.2	0.3	0.2	0.2	0.2	0.1	0.1	0.1
3-Araf	0.5	0.5	1.0	0.6	0.3	0.5	-	0.2	0.2
3-Arap or 5-Araf	3.2	2.4	2.5	2.2	0.8	1.3	0.2	0.2	0.6
3,4-Arap or 3,5-Araf	1.5	1.3	2.1	-	-	-	-	-	-
t-Rhap	0.9	0.6	1.4	1.9	1.0	1.4	0.2	0.4	0.6
2-Rhap	2.1	1.2	1.5	3.1	1.6	2.1	0.7	0.5	0.4
3-Rhap	-	-	-	-	-	-	1.6	0.9	0.8
2,4-Rhap	1.1	1.0	1.4	0.8	0.7	0.8	0.5	1.0	1.0
t-Fucp	0.3	0.2	0.4	0.6	0.4	0.8	0.2	0.5	0.5
t-Xylp	0.4	0.4	0.9	1.6	1.3	1.8	0.9	0.6	0.8
4-Xylp	6.5	9.0	12.5	20.5	21.8	22.1	59.3	38.9	32.3
2,4-Xylp	0.9	1.3	1.5	1.5	1.6	1.6	9.2	5.3	4.1
t-Manp	1.2	1.2	2.2	1.8	1.8	2.0	-	-	-
4-Manp	17.8	22.9	19.2	19.6	20.1	20.9	6.0	11.0	15.0
4,6-Manp	0.7	0.9	0.5	-	-	-	-	-	-
t-GlcAp	-	-	2.0	1.6	1.7	2.3	8.5	6.8	3.7
2-GlcAp	0.1	0.2	0.2	0.2	0.2	0.2	-	-	-
t-GalAp	1.1	0.4	0.5	2.0	1.0	1.4	-	-	-
2-GalAp	-	-	0.2	0.9	0.7	1.0	1.8	0.9	0.6
4-GalAp	23.8	13.7	4.2	15.5	12.3	8.7	1.5	0.4	0.1
3,4-GalAp	-	-	-	0.4	-	0.7	-	-	-
2,4-GalAp	0.1	0.1	0.1	0.1	-	-	-	-	0.1
t-Galp	2.9	2.1	2.7	3.2	3.1	3.0	1.2	2.9	3.1
2-Galp	0.4	0.2	0.3	0.6	-	-	-	0.4	0.6
4-Galp	-	-	3.3	-	-	2.8	-	-	0.1
6-Galp	0.4	1.1	0.8	-	-	0.8	0.5	0.8	1.0
3,4-Galp	-	1.2	1.0	0.3	-	-	-	-	-
2,4-Galp	0.2	0.3	0.3	0.2	-	-	-	0.1	0.2
t-Glcp	3.3	3.4	4.3	3.6	2.9	3.2	-	2.6	2.8
2-Glcp	0.3	0.3	0.5	0.1	0.1	0.2	-	-	-
4-Glcp	26.2	31.6	28.3	12.9	25.5	17.7	7.4	24.4	29.8
4,6-Glcp	0.4	0.7	0.6	-	-	-	-	0.4	0.7

All numbers are mole percentages. A dash designates that the amount of sugar was below detection limits. Glycosyl linkage analyses were provided by the CCRC Analytical Services.

ISSN: 2731-3654