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Table 5 Glycosyl linkage analysis of fractionated cell wall fractions from wild type P. deltoides and PdGAUT12.1 -KD lines

From: Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

Fractions Ammonium oxalate soluble Na 2 CO 3 soluble 1 M KOH soluble
WT AB30.1 AB30.8 WT AB30.1 AB30.8 WT AB30.1 AB30.8
t-Araf 3.6 1.5 3.3 3.9 1.2 2.6 0.2 0.6 0.9
t-Arap 0.2 0.2 0.3 0.2 0.2 0.2 0.1 0.1 0.1
3-Araf 0.5 0.5 1.0 0.6 0.3 0.5 - 0.2 0.2
3-Arap or 5-Araf 3.2 2.4 2.5 2.2 0.8 1.3 0.2 0.2 0.6
3,4-Arap or 3,5-Araf 1.5 1.3 2.1 - - - - - -
t-Rhap 0.9 0.6 1.4 1.9 1.0 1.4 0.2 0.4 0.6
2-Rhap 2.1 1.2 1.5 3.1 1.6 2.1 0.7 0.5 0.4
3-Rhap - - - - - - 1.6 0.9 0.8
2,4-Rhap 1.1 1.0 1.4 0.8 0.7 0.8 0.5 1.0 1.0
t-Fucp 0.3 0.2 0.4 0.6 0.4 0.8 0.2 0.5 0.5
t-Xylp 0.4 0.4 0.9 1.6 1.3 1.8 0.9 0.6 0.8
4-Xylp 6.5 9.0 12.5 20.5 21.8 22.1 59.3 38.9 32.3
2,4-Xylp 0.9 1.3 1.5 1.5 1.6 1.6 9.2 5.3 4.1
t-Manp 1.2 1.2 2.2 1.8 1.8 2.0 - - -
4-Manp 17.8 22.9 19.2 19.6 20.1 20.9 6.0 11.0 15.0
4,6-Manp 0.7 0.9 0.5 - - - - - -
t-GlcAp - - 2.0 1.6 1.7 2.3 8.5 6.8 3.7
2-GlcAp 0.1 0.2 0.2 0.2 0.2 0.2 - - -
t-GalAp 1.1 0.4 0.5 2.0 1.0 1.4 - - -
2-GalAp - - 0.2 0.9 0.7 1.0 1.8 0.9 0.6
4-GalAp 23.8 13.7 4.2 15.5 12.3 8.7 1.5 0.4 0.1
3,4-GalAp - - - 0.4 - 0.7 - - -
2,4-GalAp 0.1 0.1 0.1 0.1 - - - - 0.1
t-Galp 2.9 2.1 2.7 3.2 3.1 3.0 1.2 2.9 3.1
2-Galp 0.4 0.2 0.3 0.6 - - - 0.4 0.6
4-Galp - - 3.3 - - 2.8 - - 0.1
6-Galp 0.4 1.1 0.8 - - 0.8 0.5 0.8 1.0
3,4-Galp - 1.2 1.0 0.3 - - - - -
2,4-Galp 0.2 0.3 0.3 0.2 - - - 0.1 0.2
t-Glcp 3.3 3.4 4.3 3.6 2.9 3.2 - 2.6 2.8
2-Glcp 0.3 0.3 0.5 0.1 0.1 0.2 - - -
4-Glcp 26.2 31.6 28.3 12.9 25.5 17.7 7.4 24.4 29.8
4,6-Glcp 0.4 0.7 0.6 - - - - 0.4 0.7
  1. All numbers are mole percentages. A dash designates that the amount of sugar was below detection limits. Glycosyl linkage analyses were provided by the CCRC Analytical Services.