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Table 2 Correlations for lipid measurement obtained with Nile red or BODIPY fluorescence intensity and gravimetrical determination

From: The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae

Class

Order

Species

NR

BODIPY

Treatment

Correlation

Reference

Cyanophyceae

Synechococcales

Synechococcus sp.

-

X

-

-

[43]

Bacillariophyceae

Naviculales

Phaeodactylum tricornutum

X

-

-

-

[44]

-

X

Ethanol

-

[44]

Chlorophyceae

Chlamydomonadales

Chlamydomonas reinhardtii

X

X

Glycerol/DMSO

-

[45]

Chlamydomonas sp.

-

X

-

-

[46]

Dunaliella primolecta

X

-

DMSO

-

[25]

Dunaliella salina

X

-

-

R 2 = 0.90 (FL2/FL3)

[47]

Dunaliella tertiolecta

-

X

Glycerol/DMSO

-

[48]

Sphaeropleales

Scenedesmus dimorphus

X

-

-

R 2 = 0.9912

[49]

Scenedesmus obliquus

X

-

-

-

[50]

Scenedesmus sp.

-

X

-

-

[51]

X

-

-

R 2 = 0.99

[52]

X

-

Ultrasonic processor

-

[53]

 

Desmodesmus sp.

X

-

 

R 2 = 0.972

[54]

Coccolithophyceae

Prymnesiales

Chrysochromulina sp.

-

X

-

-

[20]

Coscinodiscophyceae

Chaetocerotales

Chaetoceros calcitrans

-

X

-

-

[25]

Eustigmatophyceae

Eustigmatales

Nannochloropsis oculata

X

-

Glutaraldehyde

R = 0.856

[55]

 

-

X

Glycerol/DMSO

-

[48]

Nannochloris atomus

-

X

Glycerol/DMSO

-

[48]

Nannochloropsis gaditana

X

-

-

Linearly correlated

[41]

Nannochloropsis sp.

X

-

-

-

[23]

Crypthecodinium cohnii

X

-

Glycerol/DMSO

-

[56]

 

X

-

Algal powder

-

[57]

 

X

-

Glycerol

-

[58]

 

X

-

Glycerol

-

[59]

 

X

-

-

R 2 = 0.9336

[17]

Peridinea

Peridiniida

Tetraselmis sp.

-

X

-

-

[43]

Prasinophyceae

Chlorodendrales

Tetraselmis suecica

-

X

Glycerol/DMSO

-

[48]

  

Tetraselmis suecica

X

-

-

R 2 = 0.81

[60]

  

Tetraselmis subcordiformis

X

 

DMSO

-

[44]

   

-

X

Ethanol

-

[44]

   

X

-

-

R 2 = 0.87

[40]

   

-

X

-

R 2 = 0.93

[61]

  

Isochrysis affinis galbana

X

-

-

R = 0.99

[42]

Prymnesiophyceae

Isochrysidales

Isochrysis galbana

X

-

Glutaraldehyde

R = 0.804

[55]

  

Isochrysis galbana

X

-

-

R = 0.83 (FL2/FL3)

[62]

  

Isochrysis sp.

X

-

-

R 2 = 0.93

[63]

  

Mallomonas splendens

-

X

-

-

[20]

Synurophyceae

Synurales

Auxenochlorella protothecoides

X

-

Ethanol

R 2 = 0.888

[64]

Trebouxiophyceae

Chlorellales

Auxenochlorella protothecoides

-

X

-

-

[64]

  

Chlorella pyrenoidosa

X

-

-

R = 0.83 (FL2/FL3)

[40]

  

Chlorella saccharophila

X

-

Lyophilization

R 2 = 0.9706

[65]

  

Chlorella sorokiniana

X

-

-

-

[50]

  

Chlorella sp.

X

-

Lyophilization

R 2 = 0.99

[24]

   

X

-

Algal powder

-

[57]

  

Chlorella vulgaris

-

X

-

R 2 = 0.95

[49]

   

X

-

Electric field

-

[66]

   

X

-

DMSO

-

[39]

   

X

-

DMSO

-

[25]

 

Trebouxiales

Botryococcus braunii

X

-

-

R 2 = 0.997

[18]

Xanthophyceae

Mischococcales

Ophiocytium maius Naegeli

-

X

-

-

[20]

  1. Determination and correlation coefficients obtained with Nile red or BODIPY 505/515 staining are expressed with the associated microalgae species and treatment. The cross indicates which dye was used.