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Table 3 Correlation of PUFA, unsaturation index, neutral or polar lipid measurement obtained with Nile red fluorescence intensity and gravimetrical quantification

From: The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae

Class

Order

Species

PUFA content

Fatty acid unsaturation index

Neutral lipid

Polar lipid

Reference

Chlorophyceae

Chlamydomonadales

Dunaliella salina

R = 0.83

R = 0.84

-

-

[62]

Prasinophyceae

Chlorodendrales

Tetraselmis suecica

Prymnesiophyceae

Isochrysiales

Isochrysis galbana

Trebouxiophyceae

Chlorellales

Chlorella pyrenoidosa

Chlorophyceae

Chlamydomonadales

Dunaliella salina

R 2 = 0.90

R 2 = 0.83

-

-

[40]

Peridinea

Peridiniida

Crypthecodinium cohnii

-

-

R 2 = 0.93

R 2 = 0.83

[17]

Prasinophyceae

Chlorodendrales

Tetraselmis suecita

R 2 = 0.83

-

R 2 = 0.87

R 2 = 0.61

[41]

  1. Correlation or determination coefficients of PUFA content and fatty acid unsaturated index were estimated between gravimetric measurements and the FL3/FL2 ratio measurement of Nile red fluorescence for microalgae species or group of algae species. Determination coefficient of neutral and polar lipids was obtained with gravimetric quantification and Nile red staining.