Figure 2

Rapid screening of the secretomes of potential transformants. Colonies from transformed plates were allowed to grow in liquid medium for 3 days prior to being screened by Western blot. (A) PVDF membrane illuminated with UV light to indicate successful transfer of broth and proteins to membrane. (B) Anti-Cel7A Western blot on the membrane shown in (A), showing numerous immunoreactive transformants. (C) We identified both ‘small’ and ‘large’ colonies after allowing transformed plates to incubate for beyond 3 days. (D) SDS-PAGE coupled Western blot highlights our observations that ‘large’ colonies are overwhelmingly more likely to be true Cel7A transformants expressing protein.