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Figure 3 | Biotechnology for Biofuels

Figure 3

From: A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina

Figure 3

eno- driven Cel7A is constitutively expressed using numerous sole carbon sources and Cel6A is not expressed in glucose medium. (A-B) The same stock of JLT102A was used to inoculate media with varied carbon sources. Following 3-day growth, the secretome was analyzed via SDS-PAGE coupled Western blots. (A) Amido black staining highlights the total extracellular protein in each described media. (B) Anti-Cel7A Western blots show Cel7A expression in each described medium. (C-D) JLT102A was grown in MAG or MAL, and a time course of medium was taken for Western blot analyis using both Cel7A and Cel6A coupled with differently colored fluorescent secondary antibodies. (C) Amido black-stained PVDF membrane showing total protein as a loading control. (D) Multiplex Western blot using anti-Cel7A (red) and anti-Cel6A (green) shows constitutive eno-Cel7A expression and glucose repression of endogenous Cel6A.

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