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Figure 4 | Biotechnology for Biofuels

Figure 4

From: A microbial platform for renewable propane synthesis based on a fermentative butanol pathway

Figure 4

Propane production in pathway-engineered E. coli strains containing wild-type ADO or the ADO A134F variant enzyme. E. coli cells were either pathway-engineered to include the atoB-TPC7 route (indicated by red colour) or the nphT7-TPC7 route (indicated by green colour) and contain wither wild-type ADO or the ADOA134F variant enzyme in the absence/presence of ferredoxin (Fdx) from Synechocystis sp. PCC 6803. A detailed protocol for the pathway engineering and propane detection is included in the ‘Materials and methods’ section and with the supporting information (Additional file 1: Figure S6). Error bars are standard deviation (n = 4).

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