Propane production in pathway-engineered
strains containing wild-type ADO or the ADO
E. coli cells were either pathway-engineered to include the atoB-TPC7 route (indicated by red colour) or the nphT7-TPC7 route (indicated by green colour) and contain wither wild-type ADO or the ADOA134F variant enzyme in the absence/presence of ferredoxin (Fdx) from Synechocystis sp. PCC 6803. A detailed protocol for the pathway engineering and propane detection is included in the ‘Materials and methods’ section and with the supporting information (Additional file 1: Figure S6). Error bars are standard deviation (n = 4).