Larger scale cultures of the best performing propane-producing pathways
. The atoB-TPC7 route (indicated by red colour) and nphT7-TPC7 (indicated by green colour) engineered in ΔyqhD knockout cells in the presence of the ADOA134F variant and ferredoxin system were analysed at larger scale. The culture volume was scaled up to 400-fold to 200 mL, in a 300-mL flask sealed with airtight rubber septum. The propane accumulation for 12 h is shown. Error bars are standard deviation (n = 3).