Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

Table 2 Differential transcript levels between 8b and SS3

		Gene flanking SNP changes						Genes in glycolysis and fermentation pathway
		ZMO 0152	ZMO 0153	ZMO 0774	ZMO 0776	ZMO 0959	ZMO 0961	ZMO 1360 (pdc)	ZMO 1596 (adhB)	ZMO 1608 (eno)
Pure glucose	8b	3.63 ± 0.02	5.45 ± 0.26	3.34 ± 0.02	4.52 ± 0.15	3.73 ± 0.11	3.73 ± 0.19	5.15 ± 0.13	5.98 ± 0.27	5.05 ± 0.49
	#3	3.69 ± 0.17	4.74 ± 0.15	3.47 ± 0.11	3.88 ± 0.28	3.69 ± 0.12	3.54 ± 0.06	5.23 ± 0.07	6.08 ± 0.01	5.13 ± 0.02
	Ratio (#3/8b)	1.14	0.19	1.36	0.23	0.92	0.64	1.21	1.25	1.2
	T-test	0.34	0.0076	0.057	0.012	0.35	0.085	0.2	0.28	0.4
40% DCS	8b	3.68 ± 0.11	5.35 ± 0.03	3.43 ± 0.20	4.41 ± 0.10	3.76 ± 0.23	3.75 ± 0.06	5.34 ± 0.02	5.82 ± 0.05	4.87 ± 0.11
	#3	3.63 ± 0.16	5.06 ± 0.09	3.21 ± 0.05	4.17 ± 0.07	3.64 ± 0.12	3.53 ± 0.13	5.16 ± 0.15	5.96 ± 0.05	4.99 ± 0.12
	Ratio (#3/8b)	0.88	0.51	0.61	0.58	0.75	0.6	0.66	1.387	1.31
	T-test	0.33	0.003	0.071	0.015	0.22	0.025	0.056	0.012	0.14
8b	Ratio (DCS/)	1.14	0.8	1.23	0.77	1.07	1.04	1.54	0.7	0.66
8b	T-test	0.28	0.28	0.23	0.17	0.42	0.44	0.036	0.19	0.28
#3	Ratio (DCS/)	0.88	2.13	0.56	1.94	0.88	0.97	0.84	0.77	0.71
#3	T-test	0.35	0.015	0.011	0.078	0.3	0.43	0.24	0.009	0.052

Transcript quantification is shown (Log₁₀) for the indicated genes in either rich medium or in 40% de-acetylated corn stover hydrolysate. SS3:8b transcript ratios are shown, with the paired T-test values indicated below. Results highlighted in blue represent statistically significant values (P ≤ 0.05), and results highlighted in bold and blue represent statistically significant values with differential values near twofold or above.

ISSN: 2731-3654