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Table 1 Kinetic and stoichiometric parameters of S. stipitis PAR, POP and C 4 in bioreactor cultivations with 60% non-detoxified HSSL

From: Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Parameters

PAR

POP

C 4

μ max (h−1)

0.037

0.037

0.067

Lag phase (h)

12

10

10

Batch duration (h)

61

62

91

Final biomass (g.L−1)

1.45 ± 0.08

3.75 ± 0.14

3.57 ± 0.13

Final CFU (cells.mL−1)

7.8 × 107 ± 6.0 × 106

3.5 × 108 ± 3.0 × 107

2.4 × 108 ± 5.8 × 107

Final xylose consumed (g.L−1)

6.1

20.7

20.4

r xylose (g.L−1.h−1)

0.10

0.33

0.22

r glucose (g.L−1.h−1)

0.10

0.13

0.10

r acetic acid (g.L−1.h−1)

0.003

0.050

0.050

r ethanol (g.L−1.h−1)

0.02

0.11

0.05

[EtOH]max (g.L−1)

1.76

6.93

4.60

Y ethanol (g.g−1)a

0.13

0.26

0.16

Conversion efficiency (%)a

26

51

32

  1. aCalculated at the maximum ethanol concentration. r refers to consumption rates for xylose, glucose and acetic acid and production rate for ethanol. PAR, parental strain; POP, population.