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Table 1 Kinetic and stoichiometric parameters of S. stipitis PAR, POP and C 4 in bioreactor cultivations with 60% non-detoxified HSSL

From: Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Parameters PAR POP C 4
μ max (h−1) 0.037 0.037 0.067
Lag phase (h) 12 10 10
Batch duration (h) 61 62 91
Final biomass (g.L−1) 1.45 ± 0.08 3.75 ± 0.14 3.57 ± 0.13
Final CFU (cells.mL−1) 7.8 × 107 ± 6.0 × 106 3.5 × 108 ± 3.0 × 107 2.4 × 108 ± 5.8 × 107
Final xylose consumed (g.L−1) 6.1 20.7 20.4
r xylose (g.L−1.h−1) 0.10 0.33 0.22
r glucose (g.L−1.h−1) 0.10 0.13 0.10
r acetic acid (g.L−1.h−1) 0.003 0.050 0.050
r ethanol (g.L−1.h−1) 0.02 0.11 0.05
[EtOH]max (g.L−1) 1.76 6.93 4.60
Y ethanol (g.g−1)a 0.13 0.26 0.16
Conversion efficiency (%)a 26 51 32
  1. aCalculated at the maximum ethanol concentration. r refers to consumption rates for xylose, glucose and acetic acid and production rate for ethanol. PAR, parental strain; POP, population.