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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Identification of a laccase Glac15 from Ganoderma lucidum 77002 and its application in bioethanol production

Figure 2

Native PAGE and SDS-PAGE of laccase purification. (a) Zymography of crude and purified laccase. Laccase activity was performed using 1 mM guaiacol as substrate. Lanes: 1: crude laccase; 2: purified laccase. (b) SDS-PAGE of crude and purified laccase. Lanes: 1: crude protein extract produced by filtering the fermentation supernatant through six layers of sterile gauze; 2: crude protein extract after ultrafiltration; 3: purified Glac15 after DEAE-Sepharose FF chromatography.

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