Table 1 Summary of the purification steps of extracellular laccase Glac15 from G. lucidum 77002
Purification step | Total volume (ml) | Total activity (U) | Total Protein (mg) | Specific activity (U/mg) | Percent recovery (%) | Purification fold |
|---|---|---|---|---|---|---|
Crude culture filtrate | 1,250 | 47,600 | 3,630 | 13.1 | 100.00 | 1.00 |
Ultrafiltration | 100 | 28,000 | 1,100 | 25.5 | 58.8 | 1.94 |
DEAE-Sepharose FF | 30 | 2,600 | 14 | 186 | 5.5 | 14.19 |