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Table 1 Nutrient formulation of the Screen 1 system

From: Automated nutrient screening system enables high-throughput optimisation of microalgae production conditions

Nutrient category Nutrient Final concentration (mM)
Nitrogen NH4Cl 0, 4.2, 8.4, 12.6, 30
NaNO3 0, 4.4, 8.8, 13.2, 30
(NH2)2CO 0, 3.8, 7.5, 11.3, 15
NH4NO3 0, 3.8, 7.5, 11.3, 15
Phosphate KH2PO4 0, 2, 10
Macroelements CaCl2 · 2H2O 0.213 (0.85)
MgSO4 · 7H2Oa 0.375 (1.50)
Microelements H3BO3 a 0.184
Fe2(SO4)3 · 7H2O (*1)a 0.001
CuSO4 · 5H2Oa 0.0064
MnCl2 · H2Oa 0.0258
ZnSO4 · 7H2Oa 0.077
Na2SeO3 a 0.0001
VOSO4 · H2Oa 0.000009
Na2SiO3 · 5H2Oa 0.273
(NH4)6MoO4 · 4H2Oa 0.00089
CoCl2 · 6H2Oa 0.0067
Chelating agent Na2EDTA, pH 8.0 0.5373
Buffer Tris-HCl, pH 7.4 100
Vitamins Vitamin B1 (thiamine hydrochloride) 0.052
Vitamin B12 (cyanocobalamin) 0.0001
  1. The average nutrient concentrations based on the literature analysis (Additional file 1: Table S3) are indicated in bold fonts. Refined nutrient concentrations after iterative cycling of nutrient screens are indicated in italic fonts. aNutrient elements were prepared as Basal Medium for 5000 tests. (*1) Fe2(SO4)3 · 7H2O is prepared in 0.5360 mM Na2-EDTA, pH 8.