|
Nutrient category
|
Nutrient
|
Final concentration (mM)
|
|---|
|
Nitrogen
|
NH4Cl
|
0, 4.2, 8.4, 12.6, 30
|
|
NaNO3
|
0, 4.4, 8.8, 13.2, 30
|
|
(NH2)2CO
|
0, 3.8, 7.5, 11.3, 15
|
|
NH4NO3
|
0, 3.8, 7.5, 11.3, 15
|
|
Phosphate
|
KH2PO4
|
0, 2, 10
|
|
Macroelements
|
CaCl2 · 2H2O
|
0.213
(0.85)
|
|
MgSO4 · 7H2Oa
|
0.375
(1.50)
|
|
Microelements
|
H3BO3
a
|
0.184
|
|
Fe2(SO4)3 · 7H2O (*1)a
|
0.001
|
|
CuSO4 · 5H2Oa
|
0.0064
|
|
MnCl2 · H2Oa
|
0.0258
|
|
ZnSO4 · 7H2Oa
|
0.077
|
|
Na2SeO3
a
|
0.0001
|
|
VOSO4 · H2Oa
|
0.000009
|
|
Na2SiO3 · 5H2Oa
|
0.273
|
|
(NH4)6MoO4 · 4H2Oa
|
0.00089
|
|
CoCl2 · 6H2Oa
|
0.0067
|
|
Chelating agent
|
Na2EDTA, pH 8.0
|
0.5373
|
|
Buffer
|
Tris-HCl, pH 7.4
|
100
|
|
Vitamins
|
Vitamin B1 (thiamine hydrochloride)
|
0.052
|
|
Vitamin B12 (cyanocobalamin)
|
0.0001
|
- The average nutrient concentrations based on the literature analysis (Additional file 1: Table S3) are indicated in bold fonts. Refined nutrient concentrations after iterative cycling of nutrient screens are indicated in italic fonts. aNutrient elements were prepared as Basal Medium for 5000 tests. (*1) Fe2(SO4)3 · 7H2O is prepared in 0.5360 mM Na2-EDTA, pH 8.
