Automated nutrient screening system enables high-throughput optimisation of microalgae production conditions

Table 1 Nutrient formulation of the Screen 1 system

Nutrient category	Nutrient	Final concentration (mM)
Nitrogen	NH₄Cl	0, 4.2, 8.4, 12.6, 30
	NaNO₃	0, 4.4, 8.8, 13.2, 30
	(NH₂)₂CO	0, 3.8, 7.5, 11.3, 15
	NH₄NO₃	0, 3.8, 7.5, 11.3, 15
Phosphate	KH₂PO₄	0, 2, 10
Macroelements	CaCl₂ · 2H₂O	0.213 (0.85)
Macroelements	MgSO₄ · 7H₂O^a	0.375 (1.50)
Microelements	H₃BO₃ ^a	0.184
	Fe₂(SO₄)₃ · 7H₂O (*¹)^a	0.001
	CuSO₄ · 5H₂O^a	0.0064
	MnCl₂ · H₂O^a	0.0258
	ZnSO₄ · 7H₂O^a	0.077
	Na₂SeO₃ ^a	0.0001
	VOSO₄ · H₂O^a	0.000009
	Na₂SiO₃ · 5H₂O^a	0.273
	(NH₄)₆MoO₄ · 4H₂O^a	0.00089
	CoCl₂ · 6H₂O^a	0.0067
Chelating agent	Na₂EDTA, pH 8.0	0.5373
Buffer	Tris-HCl, pH 7.4	100
Vitamins	Vitamin B₁ (thiamine hydrochloride)	0.052
Vitamins	Vitamin B₁₂ (cyanocobalamin)	0.0001

The average nutrient concentrations based on the literature analysis (Additional file 1: Table S3) are indicated in bold fonts. Refined nutrient concentrations after iterative cycling of nutrient screens are indicated in italic fonts. ^aNutrient elements were prepared as Basal Medium for 5000 tests. (*¹) Fe₂(SO₄)₃ · 7H₂O is prepared in 0.5360 mM Na₂-EDTA, pH 8.

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