Figure 5

Fermentation of 8% glucan AFEX CSH to > 40 g/L ethanol with a high cell density of adapted strain. Fermentation batches (A) and (B) are shown for 8% glucan AFEX-pretreated corn stover hydrolyzate that was inoculated with a large population of S. stipitis AFEX CSH-tolerant colony 5 repitched from a 6% glucan batch growth during xylose utilization. Time courses of biomass (red squares), glucose (black circles and dashed line), xylose (blue circles and solid line), xylitol (purple diamonds), ethanol (green triangles), and viable cells (aqua inverted triangles) are shown.