Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Table 1 Compositions of hydrolyzates used in cultivations ^a

Component ^b	AFEX-pretreated corn stover hydrolyzate				Dilute acid-pretreated switch grass hydrolyzates (20% solids)
	6% Glucan		12% Glucan		PSGHL		SGH		SGH-N1		SGH-N2
	Mean	s	Mean	s	Mean	s	Mean	s	Mean	s	Mean	s
Glucose (g/L)	58.9	9.0	107.4	16.7	7.9	2.5	69.2	3.2	67.4	6.5	64.2	1.3
Xylose (g/L)	34.2	7.3	48.7	15.8	52.1	5.1	48.6	2.7	45.3	3.5	47.4	0.8
Arabinose (g/L)	4.3	0.6	9.5	0.4	7.6	0.9	6.1	0.3	4.4	0.7	7.6	0.2
Galactose (g/L)	3.1	0.3	5.7	0.6	3.0	0.7	5.2	0.3	5.2	0.3	5.2	0.3
Fructose (g/L)	4.0	1.7	8.2	3.0	0.9	0.4	0.7	0	0.7	0	0.7	0
Mannose (g/L)	1.0	0.1	2.0	0.2	8.9	5.1	0	0	0	0	0	0
Acetic acid (g/L)	1.8	0.2	4.7	1.0	6.1	2.8	5.4	0.4	4.3	0.6	5.8	0.3
HMF (mM)	0.3	0.5	1.1	1.5	2.8	3.1	1.8	0.2	1.1	1.3	3.6	0.5
Furfural (mM)	0.2	0.1	0.4	0.0	24.4	7.6	18.3	4.5	24.5	4.5	19.1	0.8
PAN (mg N/L)	318.7	49.9	493.2	69.1	33.7	15.6	69.9	14.7	188.0	4.0	173.0	22.0
Urea (mg N/L)	83.1	29.3	105.5	9.1	0.7	0.7	7.0	2.7	1101.0	--^c	962.0	105.0
Ammonia (mg N/L)	1,193.4	289.7	2,707.6	385.6	25.0	21.0	23.0	24.6			369.0	63.0

^aValues are reported in terms of mean and standard deviation(s) across hydrolyzates used in experiments reported.
^bAbbreviations: HMF = hydroxymethylfurfural; PAN = primary amino nitrogen; PSGHL = dilute acid pretreated switchgrass hydrolyzate liquor; SGH = switchgrass hydrolyzate; or with nutrient supplements -N1 or -N2, as described in ‘Methods’ section.
^cNo values of s since N content was calculated based on urea addition.

ISSN: 2731-3654