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Table 4 Comparative performance of superior isolates on ODM + 74.5 g/L xylose + 77.6 g/L glucose inoculated to low cell density (A 620 = 0.1) a

From: Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

  

Sugar uptake rate (g/L/h)

Ethanol productivity (g/L/h)

Yield per initial sugar (g/g)

Adaptation stress

Isolate

Glucose

Xylose

Glucose

Xylose

Ethanol

Biomass

Xylitol

AFEX CSH

33

Colony 5

1.08

A

0.38

A

0.48

A

0.14

AB

0.37

A

0.016

BC

0.018

B

AFEX CSH > E

28

2A.1.53R-E30-C3

1.09

A

0.38

A

0.48

A

0.15

A

0.37

A

0.020

AB

0.018

B

AFEX CSH > E (UV)

30

2A.1.30R2-E40-C5

1.10

A

0.38

A

0.48

A

0.13

AB

0.35

AB

0.022

A

0.029

A

AFEX CSH > E > PSGHL

13

2A.1.53R-S100E40-1

0.96

A

0.30

C

0.41

AB

0.12

ABC

0.32

BC

0.014

BC

0.011

C

AFEX CSH > E > PSGHL

16

2A.1.53R-1

1.05

A

0.27

C

0.41

AB

0.09

C

0.29

C

0.012

CD

0.015

BC

AFEX CSH > PSGHL

11

Colony 5-GP6

0.91

A

0.27

C

0.37

B

0.11

BC

0.30

C

0.010

D

0.010

C

PSGHL

3

Y-7124-S90E40-1

1.00

A

0.38

A

0.41

AB

0.15

A

0.35

AB

0.013

CD

0.026

A

PSGHL

14

Y-7124-6

0.97

A

0.34

B

0.38

B

0.13

AB

0.32

BC

0.017

BC

0.018

B

PSGHL

15

Y-7124-10

1.08

A

0.29

C

0.44

AB

0.11

BC

0.30

C

0.009

D

0.012

C

Wild

1

Y-7124

1.05

A

0.29

C

0.48

A

0.11

BC

0.34

ABC

0.019

AB

0.010

C

  1. aWith the exception of glucose uptake rate, all parameters varied significantly among isolates based on one-way ANOVA (P < 0.01). Within columns, values with no letters in common are significantly different at P < 0.05 (Student-Newman-Keuls pairwise comparison method).