Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Slininger, Patricia J; Shea-Andersh, Maureen A; Thompson, Stephanie R; Dien, Bruce S; Kurtzman, Cletus P; Balan, Venkatesh; da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E; Cotta, Michael A

doi:10.1186/s13068-015-0239-6

Biotechnology for Biofuels and Bioproducts

Table 6 Impact of acetic acid on xylose fermentation by large isolate populations (A ₆₂₀ = 50)

From: Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

A. Comparative fermentation of 75 g/L xylose in ODM with 5 to 15 g/L acetic acid by isolates precultured on xylose
Adaptation stress	Isolate		Xylose uptake rate (g/L/hA) ^a,b		Ethanol productivity (g/L/hA) ^a,b		Ethanol yield per initial sugar (g/g) ^b
AFEX CSH	33	Colony 5	0.090	A	0.0231	AB	0.26	B
AFEX CSH > E	28	2A.1.53R-E30-C3	0.078	AB	0.0197	ABC	0.23	BCD
AFEX CSH > E (UV)	30	2A.1.30R2-E40-C5	0.071	BC	0.0155	ABCDE	0.20	DE
AFEX CSH > E > PSGHL	13	2A.1.53R S100E40-1	0.069	BC	0.0249	A	0.23	BCD
AFEX CSH > E > PSGHL	16	2A.1.53R-1	0.078	AB	0.0175	ABCD	0.25	BC
AFEX CSH > PSGHL	11	Colony 5-GP6	0.066	BC	0.0141	BCDE	0.21	CD
PSGHL	3	Y-7124 S90E40-1	0.043	D	0.0080	DE	0.14	F
PSGHL	14	Y-7124-6	0.053	CD	0.0105	CDE	0.16	EF
PSGHL	15	Y-7124-10	0.045	D	0.0061	E	0.30	A
Wild	1	Y-7124	0.060	C	0.0148	BCDE	0.19	DE
	Acetic acid (g/L)
	5		0.083	A	0.0223	A	0.29	A
	10		0.065	B	0.0152	B	0.22	B
	15		0.048	C	0.0088	C	0.14	C
B. Comparative fermentation of 75 g/L xylose in ODM with 5 to 15 g/L acetic acid by isolates precultured on glucose
Adaptation stress	Isolate		Xylose uptake rate (g/L/hA) ^a,b		Ethanol productivity (g/L/hA) ^a,b		Ethanol yield per initial sugar (g/g) ^b
AFEX CSH	33	Colony 5	0.014	C	0.0027	B	0.09	C
AFEX CSH > E	28	2A.1.53R-E30-C3	0.018	C	0.0039	B	0.09	C
AFEX CSH > E (UV)	30	2A.1.30R2-E40-C5	0.029	BC	0.0094	AB	0.16	BC
AFEX CSH > E > PSGHL	13	2A.1.53R S100E40-1	0.021	C	0.0047	B	0.18	B
AFEX CSH > E > PSGHL	16	2A.1.53R-1	0.019	C	0.0044	B	0.16	BC
AFEX CSH > PSGHL	11	Colony 5-GP6	0.014	C	0.0049	B	0.15	BC
PSGHL	3	Y-7124 S90E40-1	0.058	A	0.0165	A	0.26	A
PSGHL	14	Y-7124-6	0.044	AB	0.0108	AB	0.27	A
PSGHL	15	Y-7124-10	0.050	A	0.0141	A	0.23	A
Wild	1	Y-7124	0.018	C	0.0050	B	0.13	BC
	Acetic acid (g/L)
	5		0.043	A	0.0126	A	0.24	A
	10		0.028	B	0.0071	B	0.17	B
	15		0.014	C	0.0032	C	0.10	C

^aRates are normalized relative to population density in absorbance units (A) at 620 nm.
^bParameter variations based on two-way ANOVA (isolate × acetic ) were significant (P < 0.001). Within columns, values with no letters in common are significantly different at P < 0.05 (Student-Newman-Keuls pairwise method).

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