Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Slininger, Patricia J; Shea-Andersh, Maureen A; Thompson, Stephanie R; Dien, Bruce S; Kurtzman, Cletus P; Balan, Venkatesh; da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E; Cotta, Michael A

doi:10.1186/s13068-015-0239-6

Biotechnology for Biofuels and Bioproducts

Table 7 Comparative kinetics of isolates on switchgrass hydrolyzate SGH-N2 inoculated to A ₆₂₀ = 8.4 +/− 2.5 ^a

From: Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

A. Volumetric rates during glucose or xylose consumption
Adaptation stress	Isolate		Sugar uptake rate (g/L/h)				Ethanol productivity (g/L/h)
Adaptation stress	Isolate		Glucose		Xylose		Glucose		Xylose		Overall
AFEX CSH	33	Colony 5	0.65	A	0.19	D	0.32	A	0.174	A	0.26	A
AFEX CSH > E	28	2A.1.53R-E30-C3	0.63	A	0.27	B	0.29	A	0.076	C	0.16	D
AFEX CSH > E > PSGHL	13	2A.1.53R S100E40-1	0.43	C	0.27	B	0.20	C	0.089	C	0.15	E
AFEX CSH > E > PSGHL	16	2A.1.53R-1	0.57	AB	0.23	C	0.27	AB	0.083	C	0.17	D
PSGHL	3	Y-7124 S90E40-1	0.65	A	0.16	E	0.30	A	0.140	B	0.24	B
PSGHL	14	Y-7124-6	0.62	A	0.24	C	0.28	AB	0.090	C	0.18	C
PSGHL	15	Y-7124-10	0.63	A	0.20	D	0.30	A	0.066	C	0.16	D
Wild	1	Y-7124	0.51	B	0.30	A	0.24	B	0.059	C	0.15	E
B. Normalized rates per unit absorbance (620 nm) during glucose or xylose consumption
Adaptation stress	Isolate		Sugar uptake rate (g/L/hA)				Ethanol productivity (g/L/hA)
Adaptation stress	Isolate		Glucose		Xylose		Glucose		Xylose		Overall
AFEX CSH	33	Colony 5	0.060	D	0.017	B	0.029	C	0.0161	B	0.024	B
AFEX CSH > E	28	2A.1.53R-E30-C3	0.056	D	0.017	B	0.025	CD	0.0048	DF	0.012	C
AFEX CSH > E > PSGHL	13	2A.1.53R S100E40-1	0.040	E	0.019	B	0.018	D	0.0063	CDF	0.012	C
AFEX CSH > E > PSGHL	16	2A.1.53R-1	0.082	C	0.026	A	0.039	B	0.0094	C	0.022	B
PSGHL	3	Y-7124 S90E40-1	0.152	A	0.025	A	0.072	A	0.0216	A	0.043	A
PSGHL	14	Y-7124-6	0.057	D	0.016	B	0.026	C	0.0059	CDF	0.014	C
PSGHL	15	Y-7124-10	0.089	B	0.023	A	0.042	B	0.0076	CD	0.020	B
Wild	1	Y-7124	0.037	E	0.014	B	0.017	D	0.0028	F	0.009	D
C. Yields and residuals per total initial sugar
Adaptation stress	Isolate		Yields or residuals per total initial sugar (g/g)
			Ethanol		Biomass		Xylitol		Residual xylose
AFEX CSH	33	Colony 5	0.34	A	0.0030	D	0.025	A	0.055	B
AFEX CSH > E	28	2A.1.53R-E30-C3	0.34	A	0.0134	B	0.020	A	0.023	C
AFEX CSH > E > PSGHL	13	2A.1.53R S100E40-1	0.31	B	0.0067	D	0.024	A	0.060	B
AFEX CSH > E > PSGHL	16	2A.1.53R-1	0.34	A	0.0045	D	0.019	A	0.096	A
PSGHL	3	Y-7124 S90E40-1	0.32	B	0.0060	D	0.023	A	0.113	A
PSGHL	14	Y-7124-6	0.33	AB	0.0091	C	0.021	A	0.024	C
PSGHL	15	Y-7124-10	0.33	AB	0.0050	D	0.029	A	0.073	B
Wild	1	Y-7124	0.33	AB	0.0165	A	0.026	A	0.023	C

^aWith the exception of xylitol yield, all parameters varied significantly among isolates based on one-way ANOVA (P < 0.001). Within columns, values with no letters in common are significantly different at P < 0.05 (Student-Newman-Keuls pairwise comparison method).

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