Figure 2

Frequency of R57 mutations throughout GS evolution. The haploid UV mutant population (UV), generated from a single round of UV mutagenesis, and rounds 1, 3 and 5 of GS evolution (R1, R3 and R5, respectively) were PCR amplified at the R57 mutation loci and deep sequenced to determine the relative frequency of sequence reads bearing mutation (above a threshold of 1% of total sequence reads). The asterisk denotes mutations discovered that are not found in R57. UV, ultraviolet.