Figure 3

Prevalent mutation loci found in 20 individual isolates from the fifth round of GS. Twenty (R5-a-t) strains from the fifth round (R5) of GS were isolated, and the most prevalent mutation loci were PCR amplified and sequenced to determine their presence within each strain. The presence of homozygous mutation (green), heterozygous mutation (red), as determined by a mixed Sanger sequence at the nucleotide of interest, or no mutation (brown) are depicted for each strain.