Exploitation of algal-bacterial associations in a two-stage biohydrogen and biogas generation process

Table 2 Lysis conditions for total community DNA preparation

	Lysozyme ^a (μL)	10% CTAB ^b (μL)	Genomic CTAB lysis buffer ^c (μL)	Qiagen buffer ^d (μL)	Zymo buffer ^e (μL)
A	-	100	-	100	550
B	250	100	-	100	300
C	250	-	300	200	-

^a100 mg mL⁻¹ (Applichem, Barcelone, Spain). ^bCetyltrimethylammonium bromide (w/v). ^c1 M Tris-HCl 100 mL, 500 mM EDTA 50 mL, 5 M NaCl 300 mL, 10% CTAB, 20% SDS, pH = 8 (Wirth et al. [64]). ^dASL buffer from Qiagen QIAamp DNA Stool miniprep kit (51504, Qiagen, Limburg,Netherlands). ^eFrom Zymo Research Fecal DNA kit (Zymo Research, D6010).

ISSN: 2731-3654