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Table 2 Lysis conditions for total community DNA preparation

From: Exploitation of algal-bacterial associations in a two-stage biohydrogen and biogas generation process

  Lysozyme a (μL) 10% CTAB b (μL) Genomic CTAB lysis buffer c (μL) Qiagen buffer d (μL) Zymo buffer e (μL)
A - 100 - 100 550
B 250 100 - 100 300
C 250 - 300 200 -
  1. a100 mg mL−1 (Applichem, Barcelone, Spain). bCetyltrimethylammonium bromide (w/v). c1 M Tris-HCl 100 mL, 500 mM EDTA 50 mL, 5 M NaCl 300 mL, 10% CTAB, 20% SDS, pH = 8 (Wirth et al. [64]). dASL buffer from Qiagen QIAamp DNA Stool miniprep kit (51504, Qiagen, Limburg,Netherlands). eFrom Zymo Research Fecal DNA kit (Zymo Research, D6010).