Time course expression of
P. ostreatus aao1-6
genes following the addition of HMF. The expression levels of aao1-6, aad1, and vp1 were monitored by real-time RT-PCR (for primers information see Additional file 4). RNA was extracted from P. ostreatus at different time points (0.5, 2, 8, 14, and 48 h) after addition of 30 mM HMF to the media. The expression levels calculated relative to β-tubulin, as the endogenous control, and represent the expression relative to control without HMF addition. Bars indicate standard errors.