Figure 6

RES-1, RES-2, and RRG-2 indirectly affect lignocellulase gene expression. (A) Comparison of lignocellulase expression levels in Δres-1, Δres-2, and Δrrg-2 with WT. A total of 62 genes were grouped into three clusters: [A] cellulase genes, [B] hemicellulase genes, and [C] other genes encoding extracellular proteins. For each target, the relative proportion of transcript abundance (RPKM) is indicated as a percentage of the total. (B) Three-way Venn diagrams generated to search for TFs, which are coordinately expressed with lignocellulase genes. Top diagram: TFs induced (green arrow) by Avicel versus no carbon (top left) and versus sucrose condition (top right) and repressed (red arrow) under acute ER stress (bottom) were reasoned to potentially have a positive effect on lignocellulase gene expression. Bottom diagram: In addition, TFs being repressed when exposed to Avicel versus sucrose treatment while being induced upon ER stress could likely exert a negative effect on lignocellulase gene expression.