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Figure 1 | Biotechnology for Biofuels

Figure 1

From: Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei

Figure 1

Expression of the tcu1 gene was influenced by copper availability in T. reesei. (A) qRT-PCR analysis of the endogenous tcu1 mRNA after strain QM9414 was cultured for 12 h with 1% glucose as carbon source and with different concentrations of CuSO4. A significant difference (P < 0.05) was calculated with a Student’s t test for the transcription of tcu1 between concentrations from 0 to 200 nM of copper and those above 500 nM. Levels of endogenous tcu1 mRNA from strain QM9414 cultured in the presence of 10 μM CuSO4 with 1% glycerol (B) and 1% Avicel (C) as the sole carbon source, respectively. A significant difference (P < 0.05) existed in the transcription of tcu1 at 12 h. (D) The kinetics of turnoff of tcu1 after 10 μM CuSO4 addition. A significant difference (P < 0.05) existed in the transcription of tcu1 after adding copper for 15 min compared to 0 min. (E) qRT-PCR analysis of tcu1 mRNA after the indicated concentrations of BCS were added to a QM9414 culture for 12 h in the presence of 1 μM CuSO4. A significant difference (P < 0.05) existed for the transcription of tcu1 with and without 100 μΜ BCS. Error bars are the SD from three biological replicates.

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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