Figure 3
Homologous expression of CEL7A and CEL7B mediated by P tcu1 . SDS-PAGE analysis of the extracellular proteins of P tcu1 -cel7b grown on 1% (wt/vol) glucose (A) or glycerol (B) with (lower panel) or without (upper panel) addition of CuSO4. The extracellular CMC and pNPC hydrolytic activities of P tcu1 -cel7b and its parental strain Δcel7a grown on 1% (wt/vol) glucose (C) or glycerol (D). Error bars are the SD from two biological replicates. (E) qRT-PCR analysis of the cel7b mRNA of P tcu1 -cel7b cultured with 1% (wt/vol) glucose or 1% (wt/vol) glycerol in the presence or absence of 10 μM CuSO4. A significant difference (P < 0.05) existed for the transcription of cel7b with and without 10 μΜ Cu2+. Error bars are the SD from three biological replicates. (F) The extracellular pNPC hydrolytic activities of P tcu1 -cel7b in the presence of different concentrations of copper sulfate. (G) SDS-PAGE and Western blot analysis of the extracellular production of CEL7A by P tcu1 -cel7a grown on 1% (wt/vol) glucose with (right panel) or without (left panel) addition of CuSO4. Equal amount of culture supernatant relative to biomass was measured for all the assays.