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Figure 4 | Biotechnology for Biofuels

Figure 4

From: Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei

Figure 4

P tcu1 -xyr1 recovered cellulase gene expression on induction by 1% Avicel. SDS-PAGE and Western blot analysis of the secreted proteins in the culture supernatant of QM9414 (A) and P tcu1 -xyr1 (B) strains grown on 1% (wt/vol) Avicel with (lower panel) or without (upper panel) addition of CuSO4. (C) pNPC and pNPG hydrolytic activities of the culture filtrates from QM9414 and P tcu1 -xyr1 strains induced with 1% (wt/vol) Avicel and cultured in the presence or absence of CuSO4. A significant difference (P < 0.05) was detected for the culture filtrates of P tcu1 -xyr1 after 60 h with and without 10 μM CuSO4. Equal amount of culture supernatant relative to biomass was measured for all the assays. Error bars are the SD from at least two biological replicates.

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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