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Figure 5 | Biotechnology for Biofuels

Figure 5

From: Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei

Figure 5

P tcu1 -xyr1 recovered cellulase gene expression on induction by 1% lactose. (A) SDS-PAGE and Western blot analysis of the secreted proteins in the culture supernatant of QM9414 (A) and P tcu1 -xyr1 (B) strains grown on 1% (wt/vol) lactose with (lower panel) or without (upper panel) addition of CuSO4. (C) pNPC and pNPG hydrolytic activities of the culture filtrates from QM9414 and P tcu1 -xyr1 strains induced with 1% (wt/vol) lactose and cultured in the presence or absence of CuSO4. A significant difference (P < 0.05) was detected for the culture filtrates of P tcu1 -xyr1 after 72 h without copper addition compared with those of QM9414. Error bars are the SD from at least two biological replicates.

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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