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Figure 5 | Biotechnology for Biofuels

Figure 5

From: A new generation of versatile chromogenic substrates for high-throughput analysis of biomass-degrading enzymes

Figure 5

Multiplexed assays using the CPH substrates in different substrate and enzyme concentrations. (A) Setup of the reaction plate: CPH substrates used in the experiment were red CPH-β-glucan, green CPH-galactomannan, yellow CPH-xylan and blue CPH-arabinoxylan, and the distribution of these substrates within the reaction plate is shown to the left. Each well contained a single substrate or two, three or four substrates mixed together. Note that the total volume of substrate was the same in each well, and when substrates were mixed in wells, they were present in equal amounts. The distribution of enzymes in the reaction plate is shown to the right, and the enzymes used were xylanase nz1, xylanase nz2, glucanase nz3 and mannanase nz4. Note that the total amount of enzyme was the same in each well, and when enzymes were mixed in wells, they were present in equal amounts. Some wells contain buffer only (‘Neg’). The reaction buffer used was 50 mM sodium acetate buffer, pH 5.5. (B) Image of the product plate containing the products of the reactions shown in (A) after a 10-min reaction at 50°C. (C) to (E) spectra of selected wells as shown in the legends. See Tables 1 and 2 for details of the substrates and enzyme used.

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